Hotspot

rps12-trnV

Genome · 98710-100578 · 1869 bp

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Pi 0.00802

MarkerSeek score 64.40

Primer available yes

Label rps12-trnV

Feature Metadata

label_name: rps12-trnV
feature_type: igs
region: Genome
length_bp: 1869
variable_sites: 37
pi: 0.00802
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV_p1	GCAACTTTACGTAGGGCAGA	GATGACTTCCACCACGTCAA	50.0	50.0	57.9	57.8	1930-1963 bp	1.0	81.4
rps12-trnV_p2	GCAACTTTACGTAGGGCAGA	AACTGATGACTTCCACCACG	50.0	50.0	57.9	57.8	1934-1967 bp	1.0	81.4
rps12-trnV_p3	GCAACTTTACGTAGGGCAGA	ATGACTTCCACCACGTCAAG	50.0	50.0	57.9	57.8	1929-1962 bp	1.0	81.4
rps12-trnV_p4	CGTAGGGCAGAGTTTGGTTT	GATGACTTCCACCACGTCAA	50.0	50.0	58.1	57.8	1921-1954 bp	1.0	81.3
rps12-trnV_p5	CGTAGGGCAGAGTTTGGTTT	ATGACTTCCACCACGTCAAG	50.0	50.0	58.1	57.8	1920-1953 bp	1.0	81.3