Hotspot

clpP-psbB

Genome · 72940-73390 · 451 bp

Back to Semenovia

Pi 0.00399

MarkerSeek score 54.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: Genome
length_bp: 451
variable_sites: 4
pi: 0.00399
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GCACTATACGTCAACCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.1	58.2	512-547 bp	1.0	82.2
clpP-psbB_p2	CAGGACTTCGAAAAGGCACT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.1	58.2	477-512 bp	1.0	81.8
clpP-psbB_p3	GTCAACCCAGGATGCATCTT	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	503-538 bp	1.0	81.6
clpP-psbB_p4	AGGCACTTTTGGAACACCAA	CGATACCAAGGCAAACCCAT	45.0	50.0	58.1	58.2	464-499 bp	1.0	81.6
clpP-psbB_p5	CGGGGAAATACCGTATGACC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.8	58.2	550-585 bp	1.0	81.4