Hotspot

psbE-petL

Genome · 65782-66789 · 1008 bp

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Pi 0.00569

MarkerSeek score 62.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 1008
variable_sites: 14
pi: 0.00569
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	1159-1175 bp	1.0	81.5
psbE-petL_p2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	1172-1188 bp	1.0	81.2
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	1162-1178 bp	1.0	80.9
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.8	58.0	1082-1098 bp	1.0	80.8
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	1175-1191 bp	1.0	80.6