Hotspot

petA-psbJ

Genome · 64018-65000 · 983 bp

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Pi 0.00892

MarkerSeek score 55.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 983
variable_sites: 23
pi: 0.00892
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.1	58.1	1149-1173 bp	1.0	74.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	1136-1160 bp	1.0	74.6
petA-psbJ_p3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.1	57.8	1150-1174 bp	1.0	74.1
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	1137-1161 bp	1.0	73.8
petA-psbJ_p5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	57.7	58.1	1154-1178 bp	1.0	73.2