Hotspot

psaI-ycf4

Genome · 60487-60903 · 417 bp

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Pi 0.0107

MarkerSeek score 63.30

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: Genome
length_bp: 417
variable_sites: 10
pi: 0.0107
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCAATGGCAATGGCTTCTTT	TTTTCGCGACCCCGTTATAG	45.0	50.0	57.9	58.1	513-527 bp	1.0	82.0
psaI-ycf4_p2	GCAATGGCAATGGCTTCTTT	GGATAATGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	541-555 bp	1.0	81.8
psaI-ycf4_p3	GCAATGGCAATGGCTTCTTT	AAAGGATAATGGCCCAGCAG	45.0	50.0	57.9	57.9	544-558 bp	1.0	81.7
psaI-ycf4_p4	AGTATTTCCGGCAATGGCAA	TTTTCGCGACCCCGTTATAG	45.0	50.0	58.2	58.1	523-537 bp	1.0	81.5
psaI-ycf4_p5	AGTATTTCCGGCAATGGCAA	GGATAATGGCCCAGCAGAAA	45.0	50.0	58.2	57.9	551-565 bp	1.0	81.3