Hotspot

ndhC-trnV-UAC

Genome · 51529-52622 · 1094 bp

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Pi 0.00221

MarkerSeek score 66.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 1094
variable_sites: 7
pi: 0.00221
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1207-1246 bp	1.0	82.3
ndhC-trnV-UAC_p2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1194-1233 bp	1.0	78.5
ndhC-trnV-UAC_p3	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1192-1231 bp	1.0	77.8
ndhC-trnV-UAC_p4	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.3	58.1	1202-1241 bp	1.0	77.6
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.8	58.1	1189-1228 bp	1.0	76.7