Hotspot

trnE-UUC-trnT-GGU

Genome · 31392-32167 · 776 bp

Back to Semenovia

Pi 0.00737

MarkerSeek score 55.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: Genome
length_bp: 776
variable_sites: 15
pi: 0.00737
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	816-893 bp	1.0	84.2
trnE-UUC-trnT-GGU_p2	ATGTGCGCTTGGGAAACATA	GAACCGATGACTTACGCCTT	45.0	50.0	58.2	58.0	1480-1557 bp	1.0	83.8
trnE-UUC-trnT-GGU_p3	TATGTGCGCTTGGGAAACAT	GAACCGATGACTTACGCCTT	45.0	50.0	58.2	58.0	1481-1558 bp	1.0	83.8
trnE-UUC-trnT-GGU_p4	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	60.0	50.0	58.2	58.0	851-928 bp	1.0	83.7
trnE-UUC-trnT-GGU_p5	ATGTGCGCTTGGGAAACATA	TGGTGATTCCAGAGACGACT	45.0	50.0	58.2	58.1	1794-1878 bp	0.833333	76.4