primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TGCTTTCTACCACAGCGTTT	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.571350	5889	7126	1211-1345	1.000000	81.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TTGCTTTCTACCACAGCGTT	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.571350	5889	7126	1212-1346	1.000000	81.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.1	58.5	8.1	0.0	0.672468	5889	7126	1222-1356	1.000000	80.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TGCTTTCTACCACAGCGTTT	TCGGAGGTTCGAATCCTTTC	20	20	45.0	50.0	58.0	57.3	0.0	16.9	0.705680	5889	7126	1193-1327	1.000000	80.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTGCTTTCTACCACAGCGTT	TCGGAGGTTCGAATCCTTTC	20	20	45.0	50.0	58.0	57.3	0.0	16.9	0.705680	5889	7126	1194-1328	1.000000	80.0
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9685	9865	211-261	1.000000	69.5
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9685	9865	214-264	1.000000	69.2
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9685	9865	217-267	1.000000	68.7
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9685	9865	220-270	1.000000	68.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9685	9865	215-265	1.000000	67.0
rpoC1_p1	rpoC1	1	CCGTCCTAGTTGAATCGGAG	TTGCTCGTTCGAGAACTACG	20	20	55.0	50.0	57.5	58.0	0.0	23.5	0.533966	20918	23715	2984-2993	1.000000	76.6
rpoC1_p2	rpoC1	2	CCGTCCTAGTTGAATCGGAG	ACGATCTTTGGCTCTGGAAC	20	20	55.0	50.0	57.5	57.9	0.0	0.0	0.594318	20918	23715	2967-2976	1.000000	76.1
rpoC1_p3	rpoC1	3	CCGTCCTAGTTGAATCGGAG	TTCGATTGCTCGTTCGAGAA	20	20	55.0	45.0	57.5	57.7	0.0	23.5	0.835031	20918	23715	2989-2998	1.000000	74.1
rpoC1_p4	rpoC1	4	CCGTCCTAGTTGAATCGGAG	TTTCGATTGCTCGTTCGAGA	20	20	55.0	45.0	57.5	57.7	0.0	23.5	0.835031	20918	23715	2990-2999	1.000000	74.1
rpoC1_p5	rpoC1	5	CCGTCCTAGTTGAATCGGAG	CGATTGCTCGTTCGAGAACT	20	20	55.0	50.0	57.5	58.4	0.0	23.5	0.874852	20918	23715	2987-2996	1.000000	73.8
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCACGAACCTACAAAAGCCT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.189918	26955	28182	1365-1378	1.000000	80.2
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCCACGAACCTACAAAAGCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.195241	26955	28182	1366-1379	1.000000	80.1
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCACGAACCTACAAAAGCCT	TTATTGATCAGGCGACACCC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.433374	26955	28182	1383-1396	1.000000	78.2
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCCACGAACCTACAAAAGCC	TTATTGATCAGGCGACACCC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.438696	26955	28182	1384-1397	1.000000	78.2
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCACGAACCTACAAAAGCCT	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.1	58.4	0.0	14.6	0.463281	26955	28182	1347-1360	1.000000	78.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	31392	32167	816-893	1.000000	84.2
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	ATGTGCGCTTGGGAAACATA	GAACCGATGACTTACGCCTT	20	20	45.0	50.0	58.2	58.0	9.7	0.0	0.173087	31392	32167	1480-1557	1.000000	83.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TATGTGCGCTTGGGAAACAT	GAACCGATGACTTACGCCTT	20	20	45.0	50.0	58.2	58.0	9.7	0.0	0.173087	31392	32167	1481-1558	1.000000	83.8
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.2	58.0	0.0	0.0	0.220324	31392	32167	851-928	1.000000	83.7
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	ATGTGCGCTTGGGAAACATA	TGGTGATTCCAGAGACGACT	20	20	45.0	50.0	58.2	58.1	9.7	0.0	0.242386	31392	32167	1794-1878	0.833333	76.4
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GGCGTAAGTCATCGGTTCAA	AGACCGGACCATCCTACAAA	20	20	50.0	50.0	58.3	58.1	0.0	9.5	0.335822	32240	33733	1557-1636	1.000000	82.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GGCGTAAGTCATCGGTTCAA	GAGCAATAGACCGGACCATC	20	20	50.0	55.0	58.3	57.8	0.0	9.5	0.439149	32240	33733	1564-1643	1.000000	81.9
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	AGAGCAATAGACCGGACCAT	20	20	50.0	50.0	58.3	58.2	0.0	9.5	0.488092	32240	33733	1565-1644	1.000000	81.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.3	58.2	0.0	0.0	0.528876	32240	33733	1542-1621	1.000000	81.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	58.3	57.6	0.0	0.0	0.677352	32240	33733	1541-1620	1.000000	80.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.230316	51529	52622	1207-1246	1.000000	82.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.4	58.1	0.0	0.0	0.715163	51529	52622	1194-1233	1.000000	78.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.806132	51529	52622	1192-1231	1.000000	77.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.3	58.1	0.0	0.0	0.828958	51529	52622	1202-1241	1.000000	77.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.8	58.1	5.2	0.0	0.936224	51529	52622	1189-1228	1.000000	76.7
psaI-ycf4_p1	psaI-ycf4	1	GCAATGGCAATGGCTTCTTT	TTTTCGCGACCCCGTTATAG	20	20	45.0	50.0	57.9	58.1	8.6	9.1	0.178308	60487	60903	513-527	1.000000	82.0
psaI-ycf4_p2	psaI-ycf4	2	GCAATGGCAATGGCTTCTTT	GGATAATGGCCCAGCAGAAA	20	20	45.0	50.0	57.9	57.9	8.6	0.0	0.175315	60487	60903	541-555	1.000000	81.8
psaI-ycf4_p3	psaI-ycf4	3	GCAATGGCAATGGCTTCTTT	AAAGGATAATGGCCCAGCAG	20	20	45.0	50.0	57.9	57.9	8.6	0.0	0.181224	60487	60903	544-558	1.000000	81.7
psaI-ycf4_p4	psaI-ycf4	4	AGTATTTCCGGCAATGGCAA	TTTTCGCGACCCCGTTATAG	20	20	45.0	50.0	58.2	58.1	9.8	9.1	0.228764	60487	60903	523-537	1.000000	81.5
psaI-ycf4_p5	psaI-ycf4	5	AGTATTTCCGGCAATGGCAA	GGATAATGGCCCAGCAGAAA	20	20	45.0	50.0	58.2	57.9	9.8	0.0	0.225771	60487	60903	551-565	1.000000	81.3
ycf4-cemA_p1	ycf4-cemA	1	CGAACAAAAAGCTGCCGAAT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.127121	61459	62125	798-820	1.000000	80.4
ycf4-cemA_p2	ycf4-cemA	2	AATCGAACAAAAAGCTGCCG	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.127121	61459	62125	801-823	1.000000	80.3
ycf4-cemA_p3	ycf4-cemA	3	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	61459	62125	779-801	1.000000	80.0
ycf4-cemA_p4	ycf4-cemA	4	TTGCGTGTACCGATTGAAGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	7.8	0.0	0.234478	61459	62125	767-789	1.000000	79.6
ycf4-cemA_p5	ycf4-cemA	5	ACAAAAAGCTGCCGAATTGG	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.230011	61459	62125	795-817	1.000000	79.5
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	64018	65000	1149-1173	1.000000	74.9
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	64018	65000	1136-1160	1.000000	74.6
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	64018	65000	1150-1174	1.000000	74.1
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	64018	65000	1137-1161	1.000000	73.8
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	64018	65000	1154-1178	1.000000	73.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.060086	65782	66789	1159-1175	1.000000	81.5
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.097701	65782	66789	1172-1188	1.000000	81.2
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	6.4	0.0	0.134501	65782	66789	1162-1178	1.000000	80.9
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.165095	65782	66789	1082-1098	1.000000	80.8
psbE-petL_p5	psbE-petL	5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.172116	65782	66789	1175-1191	1.000000	80.6
clpP-psbB_p1	clpP-psbB	1	GCACTATACGTCAACCCAGG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.301360	72940	73390	512-547	1.000000	82.2
clpP-psbB_p2	clpP-psbB	2	CAGGACTTCGAAAAGGCACT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.353057	72940	73390	477-512	1.000000	81.8
clpP-psbB_p3	clpP-psbB	3	GTCAACCCAGGATGCATCTT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.9	58.2	25.3	0.0	0.372081	72940	73390	503-538	1.000000	81.6
clpP-psbB_p4	clpP-psbB	4	AGGCACTTTTGGAACACCAA	CGATACCAAGGCAAACCCAT	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.377991	72940	73390	464-499	1.000000	81.6
clpP-psbB_p5	clpP-psbB	5	CGGGGAAATACCGTATGACC	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	57.8	58.2	0.0	0.0	0.396620	72940	73390	550-585	1.000000	81.4
rps12-trnV_p1	rps12-trnV	1	GCAACTTTACGTAGGGCAGA	GATGACTTCCACCACGTCAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.252768	98710	100578	1930-1963	1.000000	81.4
rps12-trnV_p2	rps12-trnV	2	GCAACTTTACGTAGGGCAGA	AACTGATGACTTCCACCACG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.258782	98710	100578	1934-1967	1.000000	81.4
rps12-trnV_p3	rps12-trnV	3	GCAACTTTACGTAGGGCAGA	ATGACTTCCACCACGTCAAG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.258782	98710	100578	1929-1962	1.000000	81.4
rps12-trnV_p4	rps12-trnV	4	CGTAGGGCAGAGTTTGGTTT	GATGACTTCCACCACGTCAA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.271608	98710	100578	1921-1954	1.000000	81.3
rps12-trnV_p5	rps12-trnV	5	CGTAGGGCAGAGTTTGGTTT	ATGACTTCCACCACGTCAAG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.277623	98710	100578	1920-1953	1.000000	81.3
trnV-rrn16_p1	trnV-rrn16	1	TGAGTTTCTCGACCCTTTGC	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	58.1	57.8	0.0	10.4	0.368358	100637	102448	413-2071	1.000000	83.2
trnV-rrn16_p2	trnV-rrn16	2	TTGACGTGGTGGAAGTCATC	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	57.8	57.8	0.0	10.4	0.411129	100637	102448	323-1981	1.000000	83.0
trnV-rrn16_p3	trnV-rrn16	3	CGTGGTGGAAGTCATCAGTT	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	57.8	57.8	0.0	10.4	0.417143	100637	102448	319-1977	1.000000	83.0
trnV-rrn16_p4	trnV-rrn16	4	CTTGACGTGGTGGAAGTCAT	CCGACTTGCATGTGTTAAGC	20	20	50.0	50.0	57.8	57.8	0.0	10.4	0.417143	100637	102448	324-1982	1.000000	83.0
trnV-rrn16_p5	trnV-rrn16	5	TGAGTTTCTCGACCCTTTGC	GCAGGTTCTTACGCGTTACT	20	20	50.0	50.0	58.1	58.3	0.0	20.8	0.414604	100637	102448	466-2124	1.000000	82.8
ndhF_p1	ndhF	1	TTGAGGCTCTAGATACGGGA	GGGGGTAGGATCCGGTAAAT	20	20	50.0	55.0	57.0	58.3	16.7	6.0	1.305993	113034	115271	2878-2939	1.000000	72.8
ndhF_p2	ndhF	2	AATTGGAAGGGCCTCTCTTT	GGGGGTAGGATCCGGTAAAT	20	20	45.0	55.0	56.7	58.3	6.5	6.0	1.569507	113034	115271	2522-2583	1.000000	71.1
ndhF_p3	ndhF	3	AAATTGGAAGGGCCTCTCTT	GGGGGTAGGATCCGGTAAAT	20	20	45.0	55.0	56.7	58.3	6.5	6.0	1.569507	113034	115271	2523-2584	1.000000	71.0
ndhF_p4	ndhF	4	TGAGGCTCTAGATACGGGAT	GGGGGTAGGATCCGGTAAAT	20	20	50.0	55.0	56.7	58.3	16.7	6.0	1.539463	113034	115271	2877-2938	1.000000	70.9
ndhF_p5	ndhF	5	CGAGTTTAAATTGGAAGGGCC	GGGGGTAGGATCCGGTAAAT	21	20	47.6	55.0	57.5	58.3	0.0	6.0	1.765353	113034	115271	2530-2591	1.000000	69.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.174643	116374	117292	1041-1057	1.000000	82.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.176014	116374	117292	1042-1058	1.000000	82.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGATATTGGGCAGCCTTA	CTCGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	116374	117292	1062-1078	1.000000	81.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGATATTGGGCAGCCTTA	CTCTCGCACTGCTTCCTAAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	116374	117292	1064-1080	1.000000	81.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.291090	116374	117292	1051-1067	1.000000	81.2