Hotspot

trnA-UGC

Genome · 104384-104420 · 37 bp

Back to Selenicereus

Pi 0.0541

MarkerSeek score 60.80

Primer available yes

Label trnA-UGC

Feature Metadata

label_name: trnA-UGC
feature_type: tRNA
region: Genome
length_bp: 37
variable_sites: 3
pi: 0.0541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC_p1	AGACATCCAACCCGTAATCG	TCATACATGCTCCACTTGGC	50.0	50.0	57.4	58.0	92-93 bp	1.0	78.3
trnA-UGC_p2	AGACATCCAACCCGTAATCG	CATACATGCTCCACTTGGCT	50.0	50.0	57.4	58.0	91-92 bp	1.0	78.3
trnA-UGC_p3	AGACATCCAACCCGTAATCG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.4	58.0	186-187 bp	1.0	76.9
trnA-UGC_p4	ATCCAACCCGTAATCGCAAC	TGATTTACTTCACGGGCGAG	50.0	50.0	58.6	58.0	182-183 bp	1.0	76.7
trnA-UGC_p5	CATCCAACCCGTAATCGCAA	TGATTTACTTCACGGGCGAG	50.0	50.0	58.6	58.0	183-184 bp	1.0	76.7