Hotspot

rps2

Genome · 2796-3527 · 732 bp

Back to Selenicereus

Pi 0.00733

MarkerSeek score 49.50

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: Genome
length_bp: 732
variable_sites: 8
pi: 0.00733
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TGAGATTCAGGAATAGTAACCGA	TCAATGGCCAATCCGCTTTA	39.1	45.0	56.6	57.9	882-887 bp	1.0	44.5
rps2_p2	TGAGATTCAGGAATAGTAACCGA	GGGTTGTGTATCAATGGCCA	39.1	50.0	56.6	58.2	892-897 bp	1.0	44.4
rps2_p3	TGAGATTCAGGAATAGTAACCGA	TGGCCAATCCGCTTTAGAAA	39.1	45.0	56.6	57.8	878-883 bp	1.0	44.0
rps2_p4	TGAGATTCAGGAATAGTAACCGA	TATCAATGGCCAATCCGCTT	39.1	45.0	56.6	57.6	884-889 bp	1.0	42.7
rps2_p5	TGAGATTCAGGAATAGTAACCGA	GTATCAATGGCCAATCCGCT	39.1	50.0	56.6	58.4	885-890 bp	1.0	42.5