Hotspot

rpl32

Genome · 98961-99134 · 174 bp

Back to Selenicereus

Pi 0.0345

MarkerSeek score 51.50

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: Genome
length_bp: 174
variable_sites: 9
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	AACAAAAACGTTGATGGGGA	TCTTTCACATCCAACTACACCA	40.0	40.9	56.1	57.3	248 bp	1.0	45.8
rpl32_p2	ACAAAAACGTTGATGGGGAA	TCTTTCACATCCAACTACACCA	40.0	40.9	56.1	57.3	247 bp	1.0	45.8
rpl32_p3	AACAAAAACGTTGATGGGGA	TGCATAAAGAGGGTATCGTCT	40.0	42.9	56.1	56.2	326 bp	1.0	44.6
rpl32_p4	ACAAAAACGTTGATGGGGAA	TGCATAAAGAGGGTATCGTCT	40.0	42.9	56.1	56.2	325 bp	1.0	44.6
rpl32_p5	ACAAAAACGTTGATGGGGAAA	TCTTTCACATCCAACTACACCA	38.1	40.9	56.8	57.3	247 bp	1.0	43.9