Hotspot

accD

Genome · 40823-44536 · 3714 bp

Back to Selenicereus

Pi 0.0308

MarkerSeek score 51.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 3714
variable_sites: 162
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GGGTCCTATTGAAGAGCAGC	GCCCTAGCCTCCCTAATCTT	55.0	55.0	58.0	58.3	195 bp	1.0	85.6
accD_p2	CACGGGTCCTATTGAAGAGC	GCCCTAGCCTCCCTAATCTT	55.0	55.0	58.1	58.3	198 bp	1.0	85.4
accD_p3	AGGAACTGGCTTCTGCAAAA	GCCCTAGCCTCCCTAATCTT	45.0	55.0	57.9	58.3	238 bp	1.0	84.4
accD_p4	AAGGTAAGGAGGAACTGGCT	GCCCTAGCCTCCCTAATCTT	50.0	55.0	58.0	58.3	247 bp	1.0	84.3
accD_p5	AGGTAAGGAGGAACTGGCTT	GCCCTAGCCTCCCTAATCTT	50.0	55.0	58.0	58.3	246 bp	1.0	84.3