Hotspot

atpA-atpF

Genome · 132599-132666 · 68 bp

Back to Selenicereus

Pi 0.0392

MarkerSeek score 65.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: Genome
length_bp: 68
variable_sites: 4
pi: 0.0392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CAATGCCGTCACCTACTTGA	TGACATGTTTGGTGCCATGA	50.0	45.0	57.9	58.0	223 bp	1.0	80.6
atpA-atpF_p2	TGCTAATTTCGTCTGCTCGA	TGACATGTTTGGTGCCATGA	45.0	45.0	57.4	58.0	136 bp	1.0	77.1
atpA-atpF_p3	AATGCCGTCACCTACTTGAA	TGACATGTTTGGTGCCATGA	45.0	45.0	57.1	58.0	222 bp	1.0	74.6
atpA-atpF_p4	ATGCCGTCACCTACTTGAAA	TGACATGTTTGGTGCCATGA	45.0	45.0	57.1	58.0	221 bp	1.0	74.6
atpA-atpF_p5	TGCCGTCACCTACTTGAAAT	TGACATGTTTGGTGCCATGA	45.0	45.0	57.1	58.0	220 bp	1.0	74.6