Hotspot

atpF-atpA

Genome · 68821-68888 · 68 bp

Back to Selenicereus

Pi 0.0392

MarkerSeek score 65.00

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: Genome
length_bp: 68
variable_sites: 4
pi: 0.0392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	TGACATGTTTGGTGCCATGA	CAATGCCGTCACCTACTTGA	45.0	50.0	58.0	57.9	223 bp	1.0	80.6
atpF-atpA_p2	TGACATGTTTGGTGCCATGA	TGCTAATTTCGTCTGCTCGA	45.0	45.0	58.0	57.4	136 bp	1.0	77.1
atpF-atpA_p3	TGACATGTTTGGTGCCATGA	AATGCCGTCACCTACTTGAA	45.0	45.0	58.0	57.1	222 bp	1.0	74.6
atpF-atpA_p4	TGACATGTTTGGTGCCATGA	ATGCCGTCACCTACTTGAAA	45.0	45.0	58.0	57.1	221 bp	1.0	74.6
atpF-atpA_p5	TGACATGTTTGGTGCCATGA	TGCCGTCACCTACTTGAAAT	45.0	45.0	58.0	57.1	220 bp	1.0	74.6