Hotspot

trnM-CAU-accD

Genome · 40299-40822 · 524 bp

Back to Selenicereus

Pi 0.056

MarkerSeek score 64.70

Primer available yes

Label trnM-CAU-accD

Feature Metadata

label_name: trnM-CAU-accD
feature_type: igs
region: Genome
length_bp: 524
variable_sites: 43
pi: 0.056
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-accD_p1	TACGCCTTAACGACCAATGG	GCTGCTCTTCAATAGGACCC	50.0	55.0	58.0	58.0	1417-1486 bp	1.0	83.3
trnM-CAU-accD_p2	TACGCCTTAACGACCAATGG	CTTGGTTCTTTGTCCAGCCT	50.0	50.0	58.0	58.0	1213 bp	0.333333	53.1
trnM-CAU-accD_p3	TACGCCTTAACGACCAATGG	TCTTGGTTCTTTGTCCAGCC	50.0	50.0	58.0	58.0	1214 bp	0.333333	53.1
trnM-CAU-accD_p4	TACGCCTTAACGACCAATGG	CCAGTGTGGATTACGATGCA	50.0	50.0	58.0	58.0	1167 bp	0.333333	53.0
trnM-CAU-accD_p5	TACGCCTTAACGACCAATGG	ACCAGTGTGGATTACGATGC	50.0	50.0	58.0	58.0	1168 bp	0.333333	53.0