Hotspot

trnF-GAA-rbcL

Genome · 34668-35770 · 1103 bp

Back to Selenicereus

Pi 0.0184

MarkerSeek score 61.20

Primer available yes

Label trnF-GAA-rbcL

Feature Metadata

label_name: trnF-GAA-rbcL
feature_type: igs
region: Genome
length_bp: 1103
variable_sites: 28
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-rbcL_p1	GGGATAGCTCAGCTGGTAGA	CCAGCAGTAGATACCCTCGA	55.0	55.0	58.0	58.0	1139-1212 bp	1.0	85.8
trnF-GAA-rbcL_p2	CCTCGTGTCACCAGTTCAAA	CCAGCAGTAGATACCCTCGA	50.0	55.0	58.1	58.0	1103-1176 bp	1.0	85.4
trnF-GAA-rbcL_p3	GGGATAGCTCAGCTGGTAGA	CGCAATGCTAGCAAATGGAG	55.0	50.0	58.0	57.9	1211-1284 bp	1.0	84.9
trnF-GAA-rbcL_p4	GGGATAGCTCAGCTGGTAGA	GCTGCTGCTAGTGAGGTATG	55.0	55.0	58.0	58.1	1181-1254 bp	1.0	84.7
trnF-GAA-rbcL_p5	CTGGTAGAGCAGAGGACTGA	CCAGCAGTAGATACCCTCGA	55.0	55.0	57.9	58.0	1127-1200 bp	1.0	84.7