Hotspot

trnfM-CAU-rps14

Genome · 23061-23211 · 151 bp

Back to Selenicereus

Pi 0.0177

MarkerSeek score 51.30

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: Genome
length_bp: 151
variable_sites: 4
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	AGCTAACCAGACGCTCTACT	CGAGACTTTGGGTTATCCGG	50.0	55.0	57.9	58.1	336 bp	1.0	79.4
trnfM-CAU-rps14_p2	TTATGAGCCTTGCGAGCTAC	CGAGACTTTGGGTTATCCGG	50.0	55.0	57.8	58.1	274 bp	1.0	78.9
trnfM-CAU-rps14_p3	TATGAGCCTTGCGAGCTAAC	CGAGACTTTGGGTTATCCGG	50.0	55.0	57.8	58.1	349 bp	1.0	78.6
trnfM-CAU-rps14_p4	AGCTAACCAGACGCTCTACT	TGGTTCACGCATGTTTGTTG	50.0	45.0	57.9	57.8	299 bp	1.0	78.4
trnfM-CAU-rps14_p5	TTATGAGCCTTGCGAGCTAC	TGGTTCACGCATGTTTGTTG	50.0	45.0	57.8	57.8	237 bp	1.0	77.9