Hotspot

psbE-petL

LSC · 62795-63880 · 1086 bp

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Pi 0.0392
MarkerSeek score 77.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1086
variable_sites
206
pi
0.0392
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC CCGAAATAACCTGTTATAGTAAGCA 50.0 36.0 57.8 57.0 983-1155 bp 0.956522 50.3
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC CCGAAATAACCTGTTATAGTAAGCA 55.0 36.0 58.2 57.0 978-1150 bp 0.956522 50.3
psbE-petL_p3 TCTCCTGTGCTTCCAGACAT CCGAAATAACCTGTTATAGTAAGCA 50.0 36.0 58.4 57.0 976-1148 bp 0.956522 50.3
psbE-petL_p4 TTCTCCTGTGCTTCCAGACA CCGAAATAACCTGTTATAGTAAGCA 50.0 36.0 58.6 57.0 977-1149 bp 0.956522 50.3
psbE-petL_p5 AAGAACGTTCTCCTGTGCTT CCGAAATAACCTGTTATAGTAAGCA 45.0 36.0 57.4 57.0 984-1156 bp 0.956522 50.3