Hotspot

trnT-GGU-psbD

LSC · 31462-32148 · 687 bp

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Pi 0.0513
MarkerSeek score 81.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
687
variable_sites
167
pi
0.0513
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 CATAGTACCCTACCCCCAGG CGCCTAATACACCTGCAACT 60.0 50.0 58.0 58.0 1649-1929 bp 1.0 91.6
trnT-GGU-psbD_p2 CATAGTACCCTACCCCCAGG AAACTAGGCGCAAAGAACCA 60.0 45.0 58.0 58.0 1548-1828 bp 1.0 91.6
trnT-GGU-psbD_p3 CATAGTACCCTACCCCCAGG AACTAGGCGCAAAGAACCAA 60.0 45.0 58.0 58.0 1547-1827 bp 1.0 91.6
trnT-GGU-psbD_p4 CATAGTACCCTACCCCCAGG ACCAAAACTAGGCGCAAAGA 60.0 45.0 58.0 58.0 1552-1832 bp 1.0 91.6
trnT-GGU-psbD_p5 CCCACAACCTGCTATGACTC CGCCTAATACACCTGCAACT 55.0 50.0 58.0 58.0 1389-1521 bp 0.695652 79.4