Hotspot

psbM-trnD-GUC

LSC · 29918-30362 · 445 bp

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Pi 0.0448
MarkerSeek score 80.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
445
variable_sites
97
pi
0.0448
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GGAACTAAAATGAACAGTGCAGT TCAATTGGTAAGAGCACCGC 39.1 50.0 57.6 58.6 552-586 bp 1.0 60.1
psbM-trnD-GUC_p2 AAAATGAACAGTGCAGTAGC TCAATTGGTAAGAGCACCGC 40.0 50.0 54.5 58.6 546-580 bp 1.0 59.6
psbM-trnD-GUC_p3 ACTAAAATGAACAGTGCAGTAGC TCAATTGGTAAGAGCACCGC 39.1 50.0 57.4 58.6 549-583 bp 1.0 58.7
psbM-trnD-GUC_p4 AGGAACTAAAATGAACAGTGCAG TCAATTGGTAAGAGCACCGC 39.1 50.0 57.3 58.6 553-587 bp 1.0 58.0
psbM-trnD-GUC_p5 AGGAACTAAAATGAACAGTGCA TCAATTGGTAAGAGCACCGC 36.4 50.0 56.2 58.6 553-587 bp 1.0 57.1