Hotspot

trnC-GCA-petN

LSC · 28044-28922 · 879 bp

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Pi 0.0526
MarkerSeek score 80.60
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
879
variable_sites
211
pi
0.0526
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.95
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 50.0 50.0 58.1 57.8 881-944 bp 1.0 89.8
trnC-GCA-petN_p2 TTCTATTCGGAGCAACGTGG CAGCCCAAGCAAGACTTACT 50.0 50.0 58.0 57.8 1494-1580 bp 0.956522 88.7
trnC-GCA-petN_p3 ATTCGGAGCAACGTGGATTT CAGCCCAAGCAAGACTTACT 45.0 50.0 58.2 57.8 1490-1576 bp 0.956522 87.3
trnC-GCA-petN_p4 TTCTATTCGGAGCAACGTGG ATCCGCTCTTACTCATTGGC 50.0 50.0 58.0 57.8 1873-1960 bp 0.521739 70.9
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG ATCCGCTCTTACTCATTGGC 50.0 50.0 58.1 57.8 1263-1329 bp 0.521739 70.2