Hotspot

atpH-atpI

LSC · 13875-14556 · 682 bp

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Pi 0.045
MarkerSeek score 80.10
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
682
variable_sites
142
pi
0.045
unique_haplotype_count
21
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATGGGAAGGTTGGGGGAATA TCAACCGCTATCGCTATTCG 50.0 50.0 58.0 58.0 1552-1596 bp 0.73913 79.1
atpH-atpI_p2 ATGGGAAGGTTGGGGGAATA AGTGGTTCCGATACCTGTCA 50.0 50.0 58.0 58.1 1097-1123 bp 0.695652 76.8
atpH-atpI_p3 ATGGGAAGGTTGGGGGAATA TTAGGTTCAACCGCTATCGC 50.0 50.0 58.0 58.1 1558-1602 bp 0.608696 73.3
atpH-atpI_p4 TTTGAATGGGAAGGTTGGGG TCAACCGCTATCGCTATTCG 50.0 50.0 58.0 58.0 1578-1587 bp 0.347826 54.8
atpH-atpI_p5 TTTGAATGGGAAGGTTGGGG AGTGGTTCCGATACCTGTCA 50.0 50.0 58.0 58.1 1105-1114 bp 0.347826 54.8