Hotspot

trnS-GCU-trnG-GCC

LSC · 8562-9199 · 638 bp

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Pi 0.058
MarkerSeek score 82.20
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
638
variable_sites
163
pi
0.058
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 628-753 bp 1.0 59.9
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 627-752 bp 1.0 59.9
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 642-767 bp 1.0 59.1
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 641-766 bp 1.0 59.1
trnS-GCU-trnG-GCC_p5 CAATCCGACGCTTTAGTCCA TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 629-754 bp 1.0 58.8