Hotspot

rps16-trnQ-UUG

LSC · 5842-7223 · 1382 bp

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Pi 0.0755
MarkerSeek score 80.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1382
variable_sites
448
pi
0.0755
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1130-1484 bp 1.0 90.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1141-1495 bp 1.0 89.8
rps16-trnQ-UUG_p3 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1151-1505 bp 1.0 88.1
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1152-1506 bp 1.0 88.0
rps16-trnQ-UUG_p5 ATCCACAGCTGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 1153-1507 bp 1.0 86.3