Hotspot

trnK-UUU-rps16

LSC · 4073-4751 · 679 bp

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Pi 0.0636
MarkerSeek score 80.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
679
variable_sites
194
pi
0.0636
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCGTAGCAGGCACTAATA AAAAGGGTATGTTGCTGCCA 50.0 45.0 57.9 58.0 1558-1732 bp 0.869565 86.1
trnK-UUU-rps16_p2 TCAGTCGTGGTCGTACAAAC AAAAGGGTATGTTGCTGCCA 50.0 45.0 57.9 58.0 1301-1476 bp 0.869565 85.7
trnK-UUU-rps16_p3 GGACGAATCCCTTGCTTCAT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1267-1442 bp 0.826087 84.6
trnK-UUU-rps16_p4 CGTACAAACTCTACCGACGG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.1 58.0 1426-1465 bp 0.695652 78.9
trnK-UUU-rps16_p5 GGACGAATCCCTTGCTTCAT TCTTGAGCTGTACGAAGGGA 50.0 50.0 58.0 58.1 896-1059 bp 0.565217 73.6