Hotspot

rps16

Genome · 4512-5573 · 1062 bp

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Pi 0.00107
MarkerSeek score 51.10
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
Genome
length_bp
1062
variable_sites
2
pi
0.00107
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AGAAAGAGGTAAATTGTGTCT ACCACTTTGCAATGAAAAGAGG 33.3 40.9 52.4 57.7 1169-1185 bp 0.857143 35.5
rps16_p2 AAGAAAGAGGTAAATTGTGTCT ACCACTTTGCAATGAAAAGAGG 31.8 40.9 53.3 57.7 1170-1186 bp 0.857143 35.5
rps16_p3 TCCAAATAGGATTAAGAAAGAGGT ACCACTTTGCAATGAAAAGAGG 33.3 40.9 55.2 57.7 1183-1199 bp 0.857143 35.5
rps16_p4 AGAAAGAGGTAAATTGTGTCT CCACTTTGCAATGAAAAGAGGA 33.3 40.9 52.4 57.4 1168-1184 bp 0.857143 35.5
rps16_p5 AAGAAAGAGGTAAATTGTGTCT CCACTTTGCAATGAAAAGAGGA 31.8 40.9 53.3 57.4 1169-1185 bp 0.857143 35.5