Hotspot

psbA

Genome · 75-1136 · 1062 bp

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Pi 0.000628
MarkerSeek score 39.40
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
Genome
length_bp
1062
variable_sites
2
pi
0.000628
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TGGGTATTTCTGTCTTCAAAA AATACGTGTGCTTGGGAGTC 33.3 50.0 52.9 57.9 1158 bp 1.0 44.1
psbA_p2 TTGGGTATTTCTGTCTTCAAA AATACGTGTGCTTGGGAGTC 33.3 50.0 52.9 57.9 1159 bp 1.0 44.1
psbA_p3 TGGGTATTTCTGTCTTCAAAAA AATACGTGTGCTTGGGAGTC 31.8 50.0 53.8 57.9 1158 bp 1.0 44.1
psbA_p4 TTGGGTATTTCTGTCTTCAAAA AATACGTGTGCTTGGGAGTC 31.8 50.0 53.8 57.9 1159 bp 1.0 44.1
psbA_p5 ATTGGGTATTTCTGTCTTCAA AATACGTGTGCTTGGGAGTC 33.3 50.0 52.7 57.9 1160 bp 1.0 44.1