Hotspot

rpl16-rps3

Genome · 78323-79502 · 1180 bp

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Pi 0.000969
MarkerSeek score 48.30
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
Genome
length_bp
1180
variable_sites
3
pi
0.000969
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 CCTCGGCAGGATTTTCCTTT GGGTAGACTTCCCCTCCAAA 50.0 55.0 58.2 58.3 1385-1392 bp 1.0 76.7
rpl16-rps3_p2 CCTCGGCAGGATTTTCCTTT TTCCCCTCCAAACAATTCGC 50.0 50.0 58.2 58.7 1377-1384 bp 1.0 73.4
rpl16-rps3_p3 TCGGCAGGATTTTCCTTTCA GGGTAGACTTCCCCTCCAAA 45.0 55.0 57.4 58.3 1383-1390 bp 1.0 73.3
rpl16-rps3_p4 CCTCGGCAGGATTTTCCTTT GGTAGACTTCCCCTCCAAAC 50.0 55.0 58.2 57.2 1384-1391 bp 1.0 73.1
rpl16-rps3_p5 CCTCGGCAGGATTTTCCTTT CTCCAAACAATTCGCGCTAA 50.0 45.0 58.2 57.1 1372-1379 bp 1.0 72.2