Hotspot

rps18-rps12

Genome · 66428-67632 · 1205 bp

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Pi 0.00126
MarkerSeek score 46.30
Primer available yes
Label rps18-rps12

Feature Metadata

label_name
rps18-rps12
feature_type
igs
region
Genome
length_bp
1205
variable_sites
4
pi
0.00126
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rps12_p1 AAGAAACAACAACCGGAACT GAGGAACATGTGCTAGGGTG 40.0 55.0 55.7 58.0 1277 bp 1.0 61.7
rps18-rps12_p2 AGAAACAACAACCGGAACTT GAGGAACATGTGCTAGGGTG 40.0 55.0 55.7 58.0 1276 bp 1.0 61.7
rps18-rps12_p3 AAGAAACAACAACCGGAACT GTCGAGGAACATGTGCTAGG 40.0 55.0 55.7 58.1 1280 bp 1.0 61.3
rps18-rps12_p4 AGAAACAACAACCGGAACTT GTCGAGGAACATGTGCTAGG 40.0 55.0 55.7 58.1 1279 bp 1.0 61.3
rps18-rps12_p5 AAGAAACAACAACCGGAACTT GAGGAACATGTGCTAGGGTG 38.1 55.0 56.5 58.0 1277 bp 1.0 59.9