Hotspot

trnL-UAA-trnF-GAA

Genome · 47454-47813 · 360 bp

Back to Secale

Pi 0.00238
MarkerSeek score 35.30
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
Genome
length_bp
360
variable_sites
3
pi
0.00238
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 GTTGTGAGGGTTCAAGTCCC TTTGAACTGGTGACACGAGG 55.0 50.0 58.4 58.1 449-450 bp 1.0 77.0
trnL-UAA-trnF-GAA_p2 GTTGTGAGGGTTCAAGTCCC CTGCCAGGAACCAGATTTGA 55.0 50.0 58.4 57.8 464-465 bp 1.0 75.7
trnL-UAA-trnF-GAA_p3 GTTGTGAGGGTTCAAGTCCC TCTGCCAGGAACCAGATTTG 55.0 50.0 58.4 57.8 465-466 bp 1.0 75.7
trnL-UAA-trnF-GAA_p4 GTTGTGAGGGTTCAAGTCCC TTTTCTCTGCCAGGAACCAG 55.0 50.0 58.4 57.7 470-471 bp 1.0 75.1
trnL-UAA-trnF-GAA_p5 AGTTGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.4 58.1 450-451 bp 1.0 75.0