Hotspot

trnfM-CAU-trnT-GGU

Genome · 12784-14757 · 1974 bp

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Pi 0.000724
MarkerSeek score 44.90
Primer available yes
Label trnfM-CAU-trnT-GGU

Feature Metadata

label_name
trnfM-CAU-trnT-GGU
feature_type
igs
region
Genome
length_bp
1974
variable_sites
4
pi
0.000724
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnT-GGU_p1 CCTCAAGGTTATGAGCCTCG ACGACTTACCTCTTAGCACG 55.0 50.0 57.8 57.1 2131 bp 1.0 71.0
trnfM-CAU-trnT-GGU_p2 CCTCGTGAGCTACCAAACTG ACGACTTACCTCTTAGCACG 55.0 50.0 58.3 57.1 2116 bp 1.0 70.5
trnfM-CAU-trnT-GGU_p3 CCTCAAGGTTATGAGCCTCG CGACTTACCTCTTAGCACGAG 55.0 52.4 57.8 57.9 2130 bp 1.0 69.9
trnfM-CAU-trnT-GGU_p4 CCTCGTGAGCTACCAAACTG CGACTTACCTCTTAGCACGAG 55.0 52.4 58.3 57.9 2115 bp 1.0 69.4
trnfM-CAU-trnT-GGU_p5 CCTCAAGGTTATGAGCCTCG CGACTTACCTCTTAGCACGA 55.0 50.0 57.8 56.8 2130 bp 1.0 68.7