primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TGGGTATTTCTGTCTTCAAAA	AATACGTGTGCTTGGGAGTC	21	20	33.3	50.0	52.9	57.9	0.0	0.0	6.152112	75	1136	1158-1158	1.000000	44.1
psbA_p2	psbA	2	TTGGGTATTTCTGTCTTCAAA	AATACGTGTGCTTGGGAGTC	21	20	33.3	50.0	52.9	57.9	0.0	0.0	6.152112	75	1136	1159-1159	1.000000	44.1
psbA_p3	psbA	3	TGGGTATTTCTGTCTTCAAAAA	AATACGTGTGCTTGGGAGTC	22	20	31.8	50.0	53.8	57.9	0.0	0.0	6.265356	75	1136	1158-1158	1.000000	44.1
psbA_p4	psbA	4	TTGGGTATTTCTGTCTTCAAAA	AATACGTGTGCTTGGGAGTC	22	20	31.8	50.0	53.8	57.9	0.0	0.0	6.265356	75	1136	1159-1159	1.000000	44.1
psbA_p5	psbA	5	ATTGGGTATTTCTGTCTTCAA	AATACGTGTGCTTGGGAGTC	21	20	33.3	50.0	52.7	57.9	0.0	0.0	6.389740	75	1136	1160-1160	1.000000	44.1
trnK-UUU_p1	trnK-UUU	1	CAACCAAGTCAGCCCAAAGA	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.345360	1358	3931	2750-2756	1.000000	81.3
trnK-UUU_p2	trnK-UUU	2	TCAACCAAGTCAGCCCAAAG	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.345360	1358	3931	2751-2757	1.000000	81.3
trnK-UUU_p3	trnK-UUU	3	AATGTCAACCAAGTCAGCCC	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.4	58.0	0.0	0.0	0.415734	1358	3931	2755-2761	1.000000	80.7
trnK-UUU_p4	trnK-UUU	4	GTCAACCAAGTCAGCCCAAA	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	58.6	58.0	0.0	0.0	0.637864	1358	3931	2752-2758	1.000000	79.0
trnK-UUU_p5	trnK-UUU	5	ACCAAGTCAGCCCAAAGATT	AATGTTTTGACAGAAGCGCG	20	20	45.0	45.0	57.3	58.0	0.0	0.0	0.719767	1358	3931	2748-2754	1.000000	78.3
matK_p1	matK	1	TCAACCCTTTCTTGTTTCTT	CAACACCCTGTTCTGACCAT	20	20	35.0	50.0	52.8	57.7	0.0	0.0	5.487218	1670	3208	1663-1663	1.000000	44.0
matK_p2	matK	2	TCAACCCTTTCTTGTTTCTT	AATGCAACACCCTGTTCTGA	20	20	35.0	45.0	52.8	57.6	0.0	0.0	5.573792	1670	3208	1667-1667	1.000000	44.0
matK_p3	matK	3	TCAACCCTTTCTTGTTTCTT	ATGCAACACCCTGTTCTGAC	20	20	35.0	50.0	52.8	58.4	0.0	0.0	5.601278	1670	3208	1666-1666	1.000000	44.0
matK_p4	matK	4	ACCCTTTCTTGTTTCTTAATTTCGT	CAACACCCTGTTCTGACCAT	25	20	32.0	50.0	57.7	57.7	0.0	0.0	5.619501	1670	3208	1660-1660	1.000000	44.0
matK_p5	matK	5	ACCCTTTCTTGTTTCTTAATTTCGT	AATGCAACACCCTGTTCTGA	25	20	32.0	45.0	57.7	57.6	0.0	0.0	5.706076	1670	3208	1664-1664	1.000000	44.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCATAATCGTGGGCAAATGC	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.175153	3209	3893	898-904	1.000000	82.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	CATAATCGTGGGCAAATGCA	AATGTTTTGACAGAAGCGCG	20	20	45.0	45.0	57.1	58.0	7.8	0.0	0.903843	3209	3893	897-903	1.000000	76.9
matK-trnK-UUU_p3	matK-trnK-UUU	3	CCATAATCGTGGGCAAATGC	TGGGTTGCTAACTCAATGGT	20	20	50.0	45.0	57.9	57.0	0.0	0.0	1.108417	3209	3893	822-828	1.000000	75.2
matK-trnK-UUU_p4	matK-trnK-UUU	4	CCATAATCGTGGGCAAATGC	TCAATGTTTTGACAGAAGCGC	20	21	50.0	42.9	57.9	58.0	0.0	0.0	1.139824	3209	3893	900-906	1.000000	75.0
matK-trnK-UUU_p5	matK-trnK-UUU	5	CCATAATCGTGGGCAAATGC	TCTGGGTTGCTAACTCAATGG	20	21	50.0	47.6	57.9	57.9	0.0	3.7	1.210470	3209	3893	824-830	1.000000	74.4
rps16_p1	rps16	1	AGAAAGAGGTAAATTGTGTCT	ACCACTTTGCAATGAAAAGAGG	21	22	33.3	40.9	52.4	57.7	0.0	23.2	8.948890	4512	5573	1169-1185	0.857143	35.5
rps16_p2	rps16	2	AAGAAAGAGGTAAATTGTGTCT	ACCACTTTGCAATGAAAAGAGG	22	22	31.8	40.9	53.3	57.7	0.0	23.2	9.035582	4512	5573	1170-1186	0.857143	35.5
rps16_p3	rps16	3	TCCAAATAGGATTAAGAAAGAGGT	ACCACTTTGCAATGAAAAGAGG	24	22	33.3	40.9	55.2	57.7	0.0	23.2	9.085249	4512	5573	1183-1199	0.857143	35.5
rps16_p4	rps16	4	AGAAAGAGGTAAATTGTGTCT	CCACTTTGCAATGAAAAGAGGA	21	22	33.3	40.9	52.4	57.4	0.0	21.2	9.211602	4512	5573	1168-1184	0.857143	35.5
rps16_p5	rps16	5	AAGAAAGAGGTAAATTGTGTCT	CCACTTTGCAATGAAAAGAGGA	22	22	31.8	40.9	53.3	57.4	0.0	21.2	9.298294	4512	5573	1169-1185	0.857143	35.5
trnS-GCU-psbD_p1	trnS-GCU-psbD	1	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	20	20	45.0	55.0	58.0	58.1	0.0	5.0	0.148662	7643	8703	1215-1218	1.000000	79.2
trnS-GCU-psbD_p2	trnS-GCU-psbD	2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.284276	7643	8703	1193-1196	1.000000	78.1
trnS-GCU-psbD_p3	trnS-GCU-psbD	3	GAGCGGAAAGAGAGGGATTC	GAGCAATAGGCCAGACCATC	20	20	55.0	55.0	57.8	58.1	0.0	5.0	0.343212	7643	8703	1266-1269	1.000000	77.6
trnS-GCU-psbD_p4	trnS-GCU-psbD	4	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	20	20	45.0	55.0	58.0	57.7	0.0	0.0	0.349331	7643	8703	1177-1180	1.000000	77.6
trnS-GCU-psbD_p5	trnS-GCU-psbD	5	ATTAGCAATCCGCCGCTTTA	AGGCCAGACCATCCTACAAA	20	20	45.0	50.0	58.0	58.3	0.0	5.0	0.369381	7643	8703	1208-1211	1.000000	77.4
trnfM-CAU-trnT-GGU_p1	trnfM-CAU-trnT-GGU	1	CCTCAAGGTTATGAGCCTCG	ACGACTTACCTCTTAGCACG	20	20	55.0	50.0	57.8	57.1	0.0	0.0	1.146952	12784	14757	2131-2131	1.000000	71.0
trnfM-CAU-trnT-GGU_p2	trnfM-CAU-trnT-GGU	2	CCTCGTGAGCTACCAAACTG	ACGACTTACCTCTTAGCACG	20	20	55.0	50.0	58.3	57.1	0.0	0.0	1.200943	12784	14757	2116-2116	1.000000	70.5
trnfM-CAU-trnT-GGU_p3	trnfM-CAU-trnT-GGU	3	CCTCAAGGTTATGAGCCTCG	CGACTTACCTCTTAGCACGAG	20	21	55.0	52.4	57.8	57.9	0.0	0.0	1.285490	12784	14757	2130-2130	1.000000	69.9
trnfM-CAU-trnT-GGU_p4	trnfM-CAU-trnT-GGU	4	CCTCGTGAGCTACCAAACTG	CGACTTACCTCTTAGCACGAG	20	21	55.0	52.4	58.3	57.9	0.0	0.0	1.339481	12784	14757	2115-2115	1.000000	69.4
trnfM-CAU-trnT-GGU_p5	trnfM-CAU-trnT-GGU	5	CCTCAAGGTTATGAGCCTCG	CGACTTACCTCTTAGCACGA	20	20	55.0	50.0	57.8	56.8	0.0	0.0	1.429729	12784	14757	2130-2130	1.000000	68.7
atpF_p1	atpF	1	AAAAAGGGCGAGCGAAGTAA	TTCGGAAGCAAGGGGAAAAA	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.187842	32962	34344	1525-1533	1.000000	78.7
atpF_p2	atpF	2	AAAAAGGGCGAGCGAAGTAA	TTTCGGAAGCAAGGGGAAAA	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.187842	32962	34344	1526-1534	1.000000	78.7
atpF_p3	atpF	3	AAAAAGGGCGAGCGAAGTAA	TTTTCGGAAGCAAGGGGAAA	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.187842	32962	34344	1527-1535	1.000000	78.7
atpF_p4	atpF	4	AAAAAGGGCGAGCGAAGTAA	TTTTTCGGAAGCAAGGGGAA	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.187842	32962	34344	1528-1536	1.000000	78.7
atpF_p5	atpF	5	AAAAAGGGCGAGCGAAGTAA	TCGAAGGGTTGCCATTAGTG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.221063	32962	34344	1560-1568	1.000000	78.4
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	GTTGTGAGGGTTCAAGTCCC	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	58.4	58.1	0.0	20.1	0.446143	47454	47813	449-450	1.000000	77.0
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	GTTGTGAGGGTTCAAGTCCC	CTGCCAGGAACCAGATTTGA	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.599270	47454	47813	464-465	1.000000	75.7
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	GTTGTGAGGGTTCAAGTCCC	TCTGCCAGGAACCAGATTTG	20	20	55.0	50.0	58.4	57.8	0.0	0.0	0.599270	47454	47813	465-466	1.000000	75.7
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	GTTGTGAGGGTTCAAGTCCC	TTTTCTCTGCCAGGAACCAG	20	20	55.0	50.0	58.4	57.7	0.0	0.0	0.670979	47454	47813	470-471	1.000000	75.1
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AGTTGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.4	58.1	0.0	20.1	0.692212	47454	47813	450-451	1.000000	75.0
rbcL-psaI_p1	rbcL-psaI	1	CGCAGCTTGTGAAGTATGGA	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.3	57.9	0.0	26.9	0.351772	56220	57583	1499-1788	1.000000	77.5
rbcL-psaI_p2	rbcL-psaI	2	AGTTCGAGCCGGTAGATACT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.7	57.9	1.9	26.9	0.422537	56220	57583	1464-1753	1.000000	76.9
rbcL-psaI_p3	rbcL-psaI	3	GGCGATCAAATTCGAGTTCG	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	57.6	57.9	0.0	26.9	0.430670	56220	57583	1478-1767	1.000000	76.8
rbcL-psaI_p4	rbcL-psaI	4	TGGAAGGCGATCAAATTCGA	GTCATTGCAATTGCCGGAAA	20	20	45.0	45.0	57.6	57.9	0.0	26.9	0.472342	56220	57583	1483-1772	1.000000	76.5
rbcL-psaI_p5	rbcL-psaI	5	AGCTTGCAAATGGAGTCCTG	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.5	57.9	11.3	26.9	0.534920	56220	57583	1526-1815	1.000000	76.0
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	GCTGGTATTGCTGTGATTGG	20	20	50.0	50.0	57.9	57.1	0.0	0.0	0.909471	60949	61776	1005-1006	1.000000	73.0
petA-psbJ_p2	petA-psbJ	2	TGTTCTTCTTCGCATCCGTT	GCTGGTATTGCTGTGATTGG	20	20	45.0	50.0	57.8	57.1	0.0	0.0	1.021410	60949	61776	998-999	1.000000	72.1
petA-psbJ_p3	petA-psbJ	3	TTGTTCTTCTTCGCATCCGT	GCTGGTATTGCTGTGATTGG	20	20	45.0	50.0	57.8	57.1	0.0	0.0	1.021410	60949	61776	999-1000	1.000000	72.1
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTCGC	ACTGTAGCTGGTATTGCTGTG	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.073965	60949	61776	1011-1012	1.000000	71.6
petA-psbJ_p5	petA-psbJ	5	TGTTCTTCTTCGCATCCGTT	ACTGTAGCTGGTATTGCTGTG	20	21	45.0	47.6	57.8	58.0	0.0	0.0	1.185904	60949	61776	1004-1005	1.000000	70.8
rps18-rps12_p1	rps18-rps12	1	AAGAAACAACAACCGGAACT	GAGGAACATGTGCTAGGGTG	20	20	40.0	55.0	55.7	58.0	9.5	3.2	2.322146	66428	67632	1277-1277	1.000000	61.7
rps18-rps12_p2	rps18-rps12	2	AGAAACAACAACCGGAACTT	GAGGAACATGTGCTAGGGTG	20	20	40.0	55.0	55.7	58.0	9.5	3.2	2.322146	66428	67632	1276-1276	1.000000	61.7
rps18-rps12_p3	rps18-rps12	3	AAGAAACAACAACCGGAACT	GTCGAGGAACATGTGCTAGG	20	20	40.0	55.0	55.7	58.1	9.5	3.2	2.364413	66428	67632	1280-1280	1.000000	61.3
rps18-rps12_p4	rps18-rps12	4	AGAAACAACAACCGGAACTT	GTCGAGGAACATGTGCTAGG	20	20	40.0	55.0	55.7	58.1	9.5	3.2	2.364413	66428	67632	1279-1279	1.000000	61.3
rps18-rps12_p5	rps18-rps12	5	AAGAAACAACAACCGGAACTT	GAGGAACATGTGCTAGGGTG	21	20	38.1	55.0	56.5	58.0	9.5	3.2	2.537824	66428	67632	1277-1277	1.000000	59.9
rpl16-rps3_p1	rpl16-rps3	1	CCTCGGCAGGATTTTCCTTT	GGGTAGACTTCCCCTCCAAA	20	20	50.0	55.0	58.2	58.3	0.0	5.9	0.495439	78323	79502	1385-1392	1.000000	76.7
rpl16-rps3_p2	rpl16-rps3	2	CCTCGGCAGGATTTTCCTTT	TTCCCCTCCAAACAATTCGC	20	20	50.0	50.0	58.2	58.7	0.0	0.0	0.907477	78323	79502	1377-1384	1.000000	73.4
rpl16-rps3_p3	rpl16-rps3	3	TCGGCAGGATTTTCCTTTCA	GGGTAGACTTCCCCTCCAAA	20	20	45.0	55.0	57.4	58.3	0.0	5.9	0.912469	78323	79502	1383-1390	1.000000	73.3
rpl16-rps3_p4	rpl16-rps3	4	CCTCGGCAGGATTTTCCTTT	GGTAGACTTCCCCTCCAAAC	20	20	50.0	55.0	58.2	57.2	0.0	0.0	0.944185	78323	79502	1384-1391	1.000000	73.1
rpl16-rps3_p5	rpl16-rps3	5	CCTCGGCAGGATTTTCCTTT	CTCCAAACAATTCGCGCTAA	20	20	50.0	45.0	58.2	57.1	0.0	5.6	1.052949	78323	79502	1372-1379	1.000000	72.2
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	20	24	55.0	29.2	58.1	55.3	0.0	0.0	6.833076	104350	105263	1025-1053	1.000000	40.5
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TTGACATTGAAGTACGTTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	104350	105263	998-1026	1.000000	40.5
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTTGACATTGAAGTACGTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	104350	105263	999-1027	1.000000	40.5
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATTGAAGTACGTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	104350	105263	1000-1028	1.000000	40.5
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATTGAAGTACGTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	104350	105263	1001-1029	1.000000	40.5
ndhA_p1	ndhA	1	ACCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	21	20	47.6	50.0	58.1	57.9	13.3	0.0	1.204863	111750	113867	2259-2259	1.000000	70.5
ndhA_p2	ndhA	2	ACCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	21	20	47.6	50.0	58.1	57.8	13.3	0.0	1.270294	111750	113867	2257-2257	1.000000	70.0
ndhA_p3	ndhA	3	CCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	20	20	50.0	50.0	56.2	57.9	13.3	0.0	1.915426	111750	113867	2258-2258	1.000000	64.9
ndhA_p4	ndhA	4	ACCCAGTTACCATAGGGAAC	ATGGGGGAAGTTGATCGTTG	20	20	50.0	50.0	56.2	57.9	0.0	0.0	1.918051	111750	113867	2259-2259	1.000000	64.8
ndhA_p5	ndhA	5	CCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	20	20	50.0	50.0	56.2	57.8	13.3	0.0	1.980857	111750	113867	2256-2256	1.000000	64.3