Hotspot

rpl20

LSC · 65499-65861 · 363 bp

Back to Scytosiphon

Pi 0.00826

MarkerSeek score 45.40

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 363
variable_sites: 3
pi: 0.00826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	TCAAATCCCACCAGCTCCTA	GAATTGGAGTCGAACGAGCT	50.0	50.0	58.0	58.0	1107-1113 bp	1.0	80.6
rpl20_p2	TTGGGGGCTGTATTGGTTTC	GAATTGGAGTCGAACGAGCT	50.0	50.0	58.1	58.0	1471-1477 bp	1.0	80.2
rpl20_p3	ATGGACGTGGGTTCAAATCC	GAATTGGAGTCGAACGAGCT	50.0	50.0	58.2	58.0	1119-1125 bp	1.0	79.5
rpl20_p4	GGGCTGTATTGGTTTCGACA	GAATTGGAGTCGAACGAGCT	50.0	50.0	58.2	58.0	1467-1473 bp	1.0	79.3
rpl20_p5	TCAAATCCCACCAGCTCCTA	TTGGAGTCGAACGAGCTTTC	50.0	50.0	58.0	58.2	1104-1110 bp	1.0	78.8