Hotspot

dnaK

LSC · 71823-73718 · 1896 bp

Back to Scytosiphon

Pi 0.00316

MarkerSeek score 44.70

Primer available yes

Label dnaK

Feature Metadata

label_name: dnaK
feature_type: gene
region: LSC
length_bp: 1896
variable_sites: 6
pi: 0.00316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaK_p1	GCCCTGTTGAGATTATTTTAGT	AAACTAATCATAAGGATTCTCCA	36.4	30.4	54.2	52.9	2079 bp	1.0	40.3
dnaK_p2	GCCCTGTTGAGATTATTTTAGT	AACTAATCATAAGGATTCTCCAA	36.4	30.4	54.2	52.9	2078 bp	1.0	40.3
dnaK_p3	GCCCTGTTGAGATTATTTTAGT	AAACTAATCATAAGGATTCTCCAA	36.4	29.2	54.2	53.7	2079 bp	1.0	40.3
dnaK_p4	GCCCTGTTGAGATTATTTTAGTT	AAACTAATCATAAGGATTCTCCA	34.8	30.4	54.9	52.9	2079 bp	1.0	40.3
dnaK_p5	GCCCTGTTGAGATTATTTTAGTT	AACTAATCATAAGGATTCTCCAA	34.8	30.4	54.9	52.9	2078 bp	1.0	40.3