Hotspot

chlN

LSC · 111145-112512 · 1368 bp

Back to Scytosiphon

Pi 0.00365

MarkerSeek score 60.90

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: LSC
length_bp: 1368
variable_sites: 5
pi: 0.00365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	TGATTGTGTGGAAGCTGGAG	AGTTGGGTTCGAGAATACGC	50.0	50.0	57.8	58.0	2692 bp	1.0	78.6
chlN_p2	TGATTGTGTGGAAGCTGGAG	GTTGGGTTCGAGAATACGCT	50.0	50.0	57.8	58.0	2691 bp	1.0	78.6
chlN_p3	TGATTGTGTGGAAGCTGGAG	ACTACGTCCAACCCAACCTA	50.0	50.0	57.8	58.0	2656 bp	1.0	78.5
chlN_p4	ACTTGCGGGCCTTATAGGTA	AGTTGGGTTCGAGAATACGC	50.0	50.0	58.2	58.0	2416 bp	1.0	78.5
chlN_p5	ACTTGCGGGCCTTATAGGTA	GTTGGGTTCGAGAATACGCT	50.0	50.0	58.2	58.0	2415 bp	1.0	78.5