Hotspot

chlI-psaM

LSC · 104015-104087 · 73 bp

Back to Scytosiphon

Pi 0.0137

MarkerSeek score 60.40

Primer available yes

Label chlI-psaM

Feature Metadata

label_name: chlI-psaM
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 1
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI-psaM_p1	TCTTGCCCAACAATAGCAGT	GCATTCCGTTTAGGCAAAACA	45.0	42.9	57.4	58.0	174 bp	1.0	67.6
chlI-psaM_p2	TCTTGCCCAACAATAGCAGT	AGCTTTCGCAATTTTACCAGC	45.0	42.9	57.4	58.1	202 bp	1.0	67.3
chlI-psaM_p3	TCTTGCCCAACAATAGCAGT	AGCATTCCGTTTAGGCAAAAC	45.0	42.9	57.4	57.7	175 bp	1.0	65.4
chlI-psaM_p4	TCTTGCCCAACAATAGCAGT	GCTTTCGCAATTTTACCAGCA	45.0	42.9	57.4	58.3	201 bp	1.0	65.1
chlI-psaM_p5	TCTTGCCCAACAATAGCAGT	GCTTTCGCAATTTTACCAGC	45.0	45.0	57.4	56.6	201 bp	1.0	64.4