Hotspot

ycf47-rpl20

LSC · 64850-65498 · 649 bp

Back to Scytosiphon

Pi 0.00616

MarkerSeek score 62.30

Primer available yes

Label ycf47-rpl20

Feature Metadata

label_name: ycf47-rpl20
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 4
pi: 0.00616
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf47-rpl20_p1	CGCGCGGAAAAATCAATTGA	AAACTTGAATGAGCCCCACA	45.0	45.0	58.4	57.6	852 bp	1.0	74.2
ycf47-rpl20_p2	CGCGCGGAAAAATCAATTGA	AACTTGAATGAGCCCCACAA	45.0	45.0	58.4	57.6	851 bp	1.0	74.2
ycf47-rpl20_p3	CGCGCGGAAAAATCAATTGA	ACTTGAATGAGCCCCACAAA	45.0	45.0	58.4	57.6	850 bp	1.0	74.2
ycf47-rpl20_p4	CGCGCGGAAAAATCAATTGA	TGAGCCCCACAAAATCCTTT	45.0	45.0	58.4	57.6	843 bp	1.0	74.1
ycf47-rpl20_p5	CGCGCGGAAAAATCAATTGA	ATGAGCCCCACAAAATCCTT	45.0	45.0	58.4	57.4	844 bp	1.0	72.2