Hotspot

psbA-ccsA

LSC · 58824-59513 · 690 bp

Back to Scytosiphon

Pi 0.0101

MarkerSeek score 64.60

Primer available yes

Label psbA-ccsA

Feature Metadata

label_name: psbA-ccsA
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 7
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-ccsA_p1	AGCAAATGTTCCCCAGTCTC	GGCTGCAGTACAAAGAAAACA	50.0	42.9	57.8	57.3	858-859 bp	1.0	65.3
psbA-ccsA_p2	GCAAATGTTCCCCAGTCTCT	GGCTGCAGTACAAAGAAAACA	50.0	42.9	57.8	57.3	857-858 bp	1.0	65.3
psbA-ccsA_p3	AGCAAATGTTCCCCAGTCTC	AGGCTGCAGTACAAAGAAAAC	50.0	42.9	57.8	57.0	859-860 bp	1.0	63.1
psbA-ccsA_p4	GCAAATGTTCCCCAGTCTCT	AGGCTGCAGTACAAAGAAAAC	50.0	42.9	57.8	57.0	858-859 bp	1.0	63.1
psbA-ccsA_p5	GCAAATGTTCCCCAGTCTCT	CCAATAGGCTGCAGTACAAAG	50.0	47.6	57.8	57.0	863-864 bp	1.0	62.6