Hotspot

rns-psbA

LSC · 56683-57740 · 1058 bp

Back to Scytosiphon

Pi 0.0378

MarkerSeek score 80.60

Primer available yes

Label rns-psbA

Feature Metadata

label_name: rns-psbA
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 40
pi: 0.0378
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-psbA_p1	CCGTACGACTTGCATGTGTA	GTTGCGATTTCTGCACCTTC	50.0	50.0	58.0	58.0	1152-1166 bp	1.0	82.5
rns-psbA_p2	CCGTACGACTTGCATGTGTA	TTGCGATTTCTGCACCTTCT	50.0	45.0	58.0	58.1	1151-1165 bp	1.0	81.9
rns-psbA_p3	GCATGTGTAAAGCATACCGC	GTTGCGATTTCTGCACCTTC	50.0	50.0	57.9	58.0	1141-1155 bp	1.0	81.8
rns-psbA_p4	CCGTACGACTTGCATGTGTA	TGGAAGTAATGCACGAACGT	50.0	45.0	58.0	57.8	1220-1234 bp	1.0	81.3
rns-psbA_p5	GCATGTGTAAAGCATACCGC	TTGCGATTTCTGCACCTTCT	50.0	45.0	57.9	58.1	1140-1154 bp	1.0	81.2