Hotspot

psbH-ftsH

SSC · 33296-33429 · 134 bp

Back to Scytosiphon

Pi 0.0299

MarkerSeek score 69.70

Primer available yes

Label psbH-ftsH

Feature Metadata

label_name: psbH-ftsH
feature_type: igs
region: SSC
length_bp: 134
variable_sites: 4
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-ftsH_p1	AGATTGGCAGAGCTTAGGGA	AGCTGAAATACAACCAGTGGT	50.0	42.9	58.1	57.5	184 bp	1.0	68.7
psbH-ftsH_p2	AGATTGGCAGAGCTTAGGGA	GCTGAAATACAACCAGTGGT	50.0	45.0	58.1	55.9	183 bp	1.0	64.4
psbH-ftsH_p3	GATTGGCAGAGCTTAGGGAA	AGCTGAAATACAACCAGTGGT	50.0	42.9	57.3	57.5	183 bp	1.0	63.9
psbH-ftsH_p4	AGATTGGCAGAGCTTAGGGA	AAGCTGAAATACAACCAGTGGT	50.0	40.9	58.1	58.2	185 bp	1.0	63.5
psbH-ftsH_p5	AGATTGGCAGAGCTTAGGGA	AGCTGAAATACAACCAGTGG	50.0	45.0	58.1	55.6	184 bp	1.0	62.0