primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbH-ftsH_p1	psbH-ftsH	1	AGATTGGCAGAGCTTAGGGA	AGCTGAAATACAACCAGTGGT	20	21	50.0	42.9	58.1	57.5	0.0	9.7	1.623796	33296	33429	184-184	1.000000	68.7
psbH-ftsH_p2	psbH-ftsH	2	AGATTGGCAGAGCTTAGGGA	GCTGAAATACAACCAGTGGT	20	20	50.0	45.0	58.1	55.9	0.0	9.7	2.168903	33296	33429	183-183	1.000000	64.4
psbH-ftsH_p3	psbH-ftsH	3	GATTGGCAGAGCTTAGGGAA	AGCTGAAATACAACCAGTGGT	20	21	50.0	42.9	57.3	57.5	0.0	9.7	2.226484	33296	33429	183-183	1.000000	63.9
psbH-ftsH_p4	psbH-ftsH	4	AGATTGGCAGAGCTTAGGGA	AAGCTGAAATACAACCAGTGGT	20	22	50.0	40.9	58.1	58.2	0.0	9.7	2.282424	33296	33429	185-185	1.000000	63.5
psbH-ftsH_p5	psbH-ftsH	5	AGATTGGCAGAGCTTAGGGA	AGCTGAAATACAACCAGTGG	20	20	50.0	45.0	58.1	55.6	0.0	7.9	2.469559	33296	33429	184-184	1.000000	62.0
rps17-rpl29_p1	rps17-rpl29	1	TCAACAGCAACTACAACGCT	CGTTTACGACGTGGAACAAA	20	20	45.0	45.0	58.1	57.0	0.0	21.2	1.046612	46476	46530	228-228	1.000000	72.3
rps17-rpl29_p2	rps17-rpl29	2	TCAACAGCAACTACAACGCT	CGTTTACGACGTGGAACAAAA	20	21	45.0	42.9	58.1	57.7	0.0	21.2	1.353328	46476	46530	228-228	1.000000	69.9
rps17-rpl29_p3	rps17-rpl29	3	TCAACAGCAACTACAACGCT	ACGACGTGGAACAAAACAATC	20	21	45.0	42.9	58.1	57.7	0.0	4.0	1.380133	46476	46530	223-223	1.000000	69.7
rps17-rpl29_p4	rps17-rpl29	4	TCAACAGCAACTACAACGCT	ACGACGTGGAACAAAACAAT	20	20	45.0	40.0	58.1	56.5	0.0	4.0	1.554443	46476	46530	223-223	1.000000	68.3
rps17-rpl29_p5	rps17-rpl29	5	TCAACAGCAACTACAACGCT	CGACGTGGAACAAAACAATCT	20	21	45.0	42.9	58.1	57.4	0.0	0.0	1.654286	46476	46530	222-222	1.000000	67.5
rpl4_p1	rpl4	1	AGCCTAGGGTCTACTAAGAATGT	AAGAAAGTGAAGAGACCGCG	23	20	43.5	50.0	57.9	58.2	7.9	0.0	3.360987	49766	50536	905-905	1.000000	53.3
rpl4_p2	rpl4	2	AGCCTAGGGTCTACTAAGAATGT	GAAAGTGAAGAGACCGCGAA	23	20	43.5	50.0	57.9	58.2	7.9	8.2	3.365943	49766	50536	903-903	1.000000	53.2
rpl4_p3	rpl4	3	AGCCTAGGGTCTACTAAGAATGT	AAGTGAAGAGACCGCGAATG	23	20	43.5	50.0	57.9	58.3	7.9	8.2	3.430718	49766	50536	901-901	1.000000	52.7
rpl4_p4	rpl4	4	AGCCTAGGGTCTACTAAGAATGT	AAAGTGAAGAGACCGCGAAT	23	20	43.5	45.0	57.9	57.5	7.9	8.2	3.602878	49766	50536	902-902	1.000000	51.4
rpl4_p5	rpl4	5	GCCTAGGGTCTACTAAGAATGT	AAGAAAGTGAAGAGACCGCG	22	20	45.5	50.0	56.4	58.2	11.7	0.0	3.783323	49766	50536	904-904	1.000000	49.9
trnI-rns_p1	trnI-rns	1	GCCATCCTGGATTTGAACCA	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	58.1	57.9	6.4	0.0	0.224675	55096	55206	240-249	1.000000	78.9
trnI-rns_p2	trnI-rns	2	GCCATCCTGGATTTGAACCA	GTAACAAGGTAGCCGTACGG	20	20	50.0	55.0	58.1	58.1	6.4	30.1	0.235379	55096	55206	225-234	1.000000	78.8
trnI-rns_p3	trnI-rns	3	GCCATCCTGGATTTGAACCA	AACCTTTTTGGAGAGAGGCG	20	20	50.0	50.0	58.1	58.1	6.4	0.0	0.251136	55096	55206	277-286	1.000000	78.6
trnI-rns_p4	trnI-rns	4	AATGGGCCATCCTGGATTTG	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	58.2	57.9	21.5	0.0	0.278659	55096	55206	245-254	1.000000	78.4
trnI-rns_p5	trnI-rns	5	CAAATGGGCCATCCTGGATT	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	58.2	57.9	21.5	0.0	0.278659	55096	55206	247-256	1.000000	78.4
rns-psbA_p1	rns-psbA	1	CCGTACGACTTGCATGTGTA	GTTGCGATTTCTGCACCTTC	20	20	50.0	50.0	58.0	58.0	18.8	0.0	0.045562	56683	57740	1152-1166	1.000000	82.5
rns-psbA_p2	rns-psbA	2	CCGTACGACTTGCATGTGTA	TTGCGATTTCTGCACCTTCT	20	20	50.0	45.0	58.0	58.1	18.8	0.0	0.118446	56683	57740	1151-1165	1.000000	81.9
rns-psbA_p3	rns-psbA	3	GCATGTGTAAAGCATACCGC	GTTGCGATTTCTGCACCTTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.136219	56683	57740	1141-1155	1.000000	81.8
rns-psbA_p4	rns-psbA	4	CCGTACGACTTGCATGTGTA	TGGAAGTAATGCACGAACGT	20	20	50.0	45.0	58.0	57.8	18.8	0.0	0.172500	56683	57740	1220-1234	1.000000	81.3
rns-psbA_p5	rns-psbA	5	GCATGTGTAAAGCATACCGC	TTGCGATTTCTGCACCTTCT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.209103	56683	57740	1140-1154	1.000000	81.2
psbA-ccsA_p1	psbA-ccsA	1	AGCAAATGTTCCCCAGTCTC	GGCTGCAGTACAAAGAAAACA	20	21	50.0	42.9	57.8	57.3	0.0	16.6	1.915139	58824	59513	858-859	1.000000	65.3
psbA-ccsA_p2	psbA-ccsA	2	GCAAATGTTCCCCAGTCTCT	GGCTGCAGTACAAAGAAAACA	20	21	50.0	42.9	57.8	57.3	0.0	16.6	1.915139	58824	59513	857-858	1.000000	65.3
psbA-ccsA_p3	psbA-ccsA	3	AGCAAATGTTCCCCAGTCTC	AGGCTGCAGTACAAAGAAAAC	20	21	50.0	42.9	57.8	57.0	0.0	16.6	2.196894	58824	59513	859-860	1.000000	63.1
psbA-ccsA_p4	psbA-ccsA	4	GCAAATGTTCCCCAGTCTCT	AGGCTGCAGTACAAAGAAAAC	20	21	50.0	42.9	57.8	57.0	0.0	16.6	2.196894	58824	59513	858-859	1.000000	63.1
psbA-ccsA_p5	psbA-ccsA	5	GCAAATGTTCCCCAGTCTCT	CCAATAGGCTGCAGTACAAAG	20	21	50.0	47.6	57.8	57.0	0.0	16.6	2.250359	58824	59513	863-864	1.000000	62.6
ycf47-rpl20_p1	ycf47-rpl20	1	CGCGCGGAAAAATCAATTGA	AAACTTGAATGAGCCCCACA	20	20	45.0	45.0	58.4	57.6	24.8	0.0	0.767909	64850	65498	852-852	1.000000	74.2
ycf47-rpl20_p2	ycf47-rpl20	2	CGCGCGGAAAAATCAATTGA	AACTTGAATGAGCCCCACAA	20	20	45.0	45.0	58.4	57.6	24.8	0.0	0.767909	64850	65498	851-851	1.000000	74.2
ycf47-rpl20_p3	ycf47-rpl20	3	CGCGCGGAAAAATCAATTGA	ACTTGAATGAGCCCCACAAA	20	20	45.0	45.0	58.4	57.6	24.8	0.0	0.767909	64850	65498	850-850	1.000000	74.2
ycf47-rpl20_p4	ycf47-rpl20	4	CGCGCGGAAAAATCAATTGA	TGAGCCCCACAAAATCCTTT	20	20	45.0	45.0	58.4	57.6	24.8	0.0	0.784888	64850	65498	843-843	1.000000	74.1
ycf47-rpl20_p5	ycf47-rpl20	5	CGCGCGGAAAAATCAATTGA	ATGAGCCCCACAAAATCCTT	20	20	45.0	45.0	58.4	57.4	24.8	0.0	1.016862	64850	65498	844-844	1.000000	72.2
rpl20_p1	rpl20	1	TCAAATCCCACCAGCTCCTA	GAATTGGAGTCGAACGAGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.036917	65499	65861	1107-1113	1.000000	80.6
rpl20_p2	rpl20	2	TTGGGGGCTGTATTGGTTTC	GAATTGGAGTCGAACGAGCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.070696	65499	65861	1471-1477	1.000000	80.2
rpl20_p3	rpl20	3	ATGGACGTGGGTTCAAATCC	GAATTGGAGTCGAACGAGCT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.168143	65499	65861	1119-1125	1.000000	79.5
rpl20_p4	rpl20	4	GGGCTGTATTGGTTTCGACA	GAATTGGAGTCGAACGAGCT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.186256	65499	65861	1467-1473	1.000000	79.3
rpl20_p5	rpl20	5	TCAAATCCCACCAGCTCCTA	TTGGAGTCGAACGAGCTTTC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.255016	65499	65861	1104-1110	1.000000	78.8
dnaK_p1	dnaK	1	GCCCTGTTGAGATTATTTTAGT	AAACTAATCATAAGGATTCTCCA	22	23	36.4	30.4	54.2	52.9	0.0	0.0	13.954443	71823	73718	2079-2079	1.000000	40.3
dnaK_p2	dnaK	2	GCCCTGTTGAGATTATTTTAGT	AACTAATCATAAGGATTCTCCAA	22	23	36.4	30.4	54.2	52.9	0.0	0.0	13.954443	71823	73718	2078-2078	1.000000	40.3
dnaK_p3	dnaK	3	GCCCTGTTGAGATTATTTTAGT	AAACTAATCATAAGGATTCTCCAA	22	24	36.4	29.2	54.2	53.7	0.0	0.0	14.130211	71823	73718	2079-2079	1.000000	40.3
dnaK_p4	dnaK	4	GCCCTGTTGAGATTATTTTAGTT	AAACTAATCATAAGGATTCTCCA	23	23	34.8	30.4	54.9	52.9	0.0	0.0	14.166397	71823	73718	2079-2079	1.000000	40.3
dnaK_p5	dnaK	5	GCCCTGTTGAGATTATTTTAGTT	AACTAATCATAAGGATTCTCCAA	23	23	34.8	30.4	54.9	52.9	0.0	0.0	14.166397	71823	73718	2078-2078	1.000000	40.3
ycf46_p1	ycf46	1	TCGTCGTCGAAGAAAAAGAA	TGCTAGGAATAATTTTAGACCAAGA	20	25	40.0	32.0	55.4	56.0	8.5	0.0	9.559583	78777	80252	1599-1599	1.000000	40.2
ycf46_p2	ycf46	2	CGTCGTCGAAGAAAAAGAAAGA	TGCTAGGAATAATTTTAGACCAAGA	22	25	40.9	32.0	57.3	56.0	1.6	0.0	9.658310	78777	80252	1598-1598	1.000000	40.2
ycf46_p3	ycf46	3	TCGTCGTCGAAGAAAAAGAAAG	TGCTAGGAATAATTTTAGACCAAGA	22	25	40.9	32.0	57.3	56.0	8.5	0.0	9.658310	78777	80252	1599-1599	1.000000	40.2
ycf46_p4	ycf46	4	TCGTCGTCGAAGAAAAAGAAA	TGCTAGGAATAATTTTAGACCAAGA	21	25	38.1	32.0	56.2	56.0	8.5	0.0	9.782436	78777	80252	1599-1599	1.000000	40.2
ycf46_p5	ycf46	5	TCGTCGTCGAAGAAAAAGAA	TGCTAGGAATAATTTTAGACCAAG	20	24	40.0	33.3	55.4	54.7	8.5	0.0	9.916887	78777	80252	1599-1599	1.000000	40.2
atpA_p1	atpA	1	TCTGTAATTCGGTTGTTTTCT	TTATTTAAGGCCTCTCTAAAGT	21	22	33.3	31.8	53.4	52.2	0.0	5.2	13.389604	99709	101214	1670-1670	1.000000	40.1
atpA_p2	atpA	2	TCTGTAATTCGGTTGTTTTCT	TTTAAGGCCTCTCTAAAGTATT	21	22	33.3	31.8	53.4	52.2	0.0	5.2	13.389604	99709	101214	1667-1667	1.000000	40.1
atpA_p3	atpA	3	TCTGTAATTCGGTTGTTTTCTT	TTATTTAAGGCCTCTCTAAAGT	22	22	31.8	31.8	54.2	52.2	0.0	5.2	13.535303	99709	101214	1670-1670	1.000000	40.1
atpA_p4	atpA	4	TCTGTAATTCGGTTGTTTTCTT	TTTAAGGCCTCTCTAAAGTATT	22	22	31.8	31.8	54.2	52.2	0.0	5.2	13.535303	99709	101214	1667-1667	1.000000	40.1
atpA_p5	atpA	5	TTCTGTAATTCGGTTGTTTTCT	TTTAAGGCCTCTCTAAAGTATT	22	22	31.8	31.8	54.2	52.2	0.0	5.2	13.535303	99709	101214	1668-1668	1.000000	40.1
chlI-psaM_p1	chlI-psaM	1	TCTTGCCCAACAATAGCAGT	GCATTCCGTTTAGGCAAAACA	20	21	45.0	42.9	57.4	58.0	0.0	12.6	1.604768	104015	104087	174-174	1.000000	67.6
chlI-psaM_p2	chlI-psaM	2	TCTTGCCCAACAATAGCAGT	AGCTTTCGCAATTTTACCAGC	20	21	45.0	42.9	57.4	58.1	0.0	0.0	1.638271	104015	104087	202-202	1.000000	67.3
chlI-psaM_p3	chlI-psaM	3	TCTTGCCCAACAATAGCAGT	AGCATTCCGTTTAGGCAAAAC	20	21	45.0	42.9	57.4	57.7	0.0	4.3	1.882665	104015	104087	175-175	1.000000	65.4
chlI-psaM_p4	chlI-psaM	4	TCTTGCCCAACAATAGCAGT	GCTTTCGCAATTTTACCAGCA	20	21	45.0	42.9	57.4	58.3	0.0	0.0	1.913240	104015	104087	201-201	1.000000	65.1
chlI-psaM_p5	chlI-psaM	5	TCTTGCCCAACAATAGCAGT	GCTTTCGCAATTTTACCAGC	20	20	45.0	45.0	57.4	56.6	0.0	0.0	2.004727	104015	104087	201-201	1.000000	64.4
chlL_p1	chlL	1	GGGGTACGAAAGGCTAGTTG	CGCGGTTCAGCAAAAATCAT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.038694	110158	111027	1434-1437	1.000000	80.2
chlL_p2	chlL	2	GGGTACGAAAGGCTAGTTGG	CGCGGTTCAGCAAAAATCAT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.038694	110158	111027	1433-1436	1.000000	80.2
chlL_p3	chlL	3	TAGTTCGTTGGCGTGACAAA	CGCGGTTCAGCAAAAATCAT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.081468	110158	111027	1933-1936	1.000000	79.8
chlL_p4	chlL	4	TTAGTTCGTTGGCGTGACAA	CGCGGTTCAGCAAAAATCAT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.081468	110158	111027	1934-1937	1.000000	79.8
chlL_p5	chlL	5	TTTAGTTCGTTGGCGTGACA	CGCGGTTCAGCAAAAATCAT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.081468	110158	111027	1935-1938	1.000000	79.8
chlN_p1	chlN	1	TGATTGTGTGGAAGCTGGAG	AGTTGGGTTCGAGAATACGC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.195564	111145	112512	2692-2692	1.000000	78.6
chlN_p2	chlN	2	TGATTGTGTGGAAGCTGGAG	GTTGGGTTCGAGAATACGCT	20	20	50.0	50.0	57.8	58.0	0.0	4.0	0.195564	111145	112512	2691-2691	1.000000	78.6
chlN_p3	chlN	3	TGATTGTGTGGAAGCTGGAG	ACTACGTCCAACCCAACCTA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.203604	111145	112512	2656-2656	1.000000	78.5
chlN_p4	chlN	4	ACTTGCGGGCCTTATAGGTA	AGTTGGGTTCGAGAATACGC	20	20	50.0	50.0	58.2	58.0	0.8	0.0	0.208650	111145	112512	2416-2416	1.000000	78.5
chlN_p5	chlN	5	ACTTGCGGGCCTTATAGGTA	GTTGGGTTCGAGAATACGCT	20	20	50.0	50.0	58.2	58.0	0.8	4.0	0.208650	111145	112512	2415-2415	1.000000	78.5
petA_p1	petA	1	ACTTGTTTCTATTAGTGCAAGCT	ACCTCAATTAATCTAATAAACAAGAGC	23	27	34.8	29.6	56.5	56.3	16.6	0.0	13.281016	115278	116225	1162-1163	1.000000	40.1
petA_p2	petA	2	ACTTGTTTCTATTAGTGCAAGCT	CCTCAATTAATCTAATAAACAAGAGCT	23	27	34.8	29.6	56.5	56.0	16.6	0.0	13.506243	115278	116225	1161-1162	1.000000	40.1
petA_p3	petA	3	ACTTGTTTCTATTAGTGCAAGCTT	ACCTCAATTAATCTAATAAACAAGAGC	24	27	33.3	29.6	57.1	56.3	16.6	0.0	13.621317	115278	116225	1162-1163	1.000000	40.1
petA_p4	petA	4	ACTTGTTTCTATTAGTGCAAGC	ACCTCAATTAATCTAATAAACAAGAGC	22	27	36.4	29.6	55.0	56.3	16.6	0.0	13.709777	115278	116225	1162-1163	1.000000	40.1
petA_p5	petA	5	ACTTGTTTCTATTAGTGCAAGCT	CCTCAATTAATCTAATAAACAAGAGC	23	26	34.8	30.8	56.5	54.8	16.6	0.0	13.735176	115278	116225	1161-1162	1.000000	40.1
trnC-psbY_p1	trnC-psbY	1	AGTTCGAATCTGAGTGTCGC	TGCAGCTTCATGGGCATTAT	20	20	50.0	45.0	58.0	57.9	0.0	12.6	0.092235	129540	130298	855-856	1.000000	80.0
trnC-psbY_p2	trnC-psbY	2	TAAGGCCGTGGATTGCAAAT	TGCAGCTTCATGGGCATTAT	20	20	45.0	45.0	58.2	57.9	1.7	12.6	0.230200	129540	130298	886-887	1.000000	78.9
trnC-psbY_p3	trnC-psbY	3	CCGTGGATTGCAAATCCTCT	TGCAGCTTCATGGGCATTAT	20	20	50.0	45.0	58.2	57.9	21.1	12.6	0.321028	129540	130298	881-882	1.000000	78.2
trnC-psbY_p4	trnC-psbY	4	TCGAATCTGAGTGTCGCCTA	TGCAGCTTCATGGGCATTAT	20	20	50.0	45.0	58.3	57.9	3.3	12.6	0.329737	129540	130298	852-853	1.000000	78.1
trnC-psbY_p5	trnC-psbY	5	AGTTCGAATCTGAGTGTCGC	CTGCAGCTTCATGGGCATTA	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.620393	129540	130298	856-857	1.000000	75.8