Hotspot

ycf1

SSC · 121787-127423 · 5637 bp

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Pi 0.0233

MarkerSeek score 66.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5637
variable_sites: 1367
pi: 0.0233
unique_haplotype_count: 170
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCTTTCCATTGACTCGGAT	GCTCGGGTTATGGAAGAAGG	50.0	55.0	58.0	58.0	680-815 bp	0.837079	84.8
ycf1_p2	GCCTTTCCATTGACTCGGAT	AGGGCCCTCTTATCTCTTCC	50.0	55.0	58.0	57.9	708-843 bp	0.837079	84.2
ycf1_p3	GCCTTTCCATTGACTCGGAT	GGGCCCTCTTATCTCTTCCT	50.0	55.0	58.0	57.9	707-842 bp	0.837079	84.2
ycf1_p4	GCCTTTCCATTGACTCGGAT	TCGTTGTGGTCGGACTCTAT	50.0	50.0	58.0	58.2	754-889 bp	0.837079	83.8
ycf1_p5	GCCTTTCCATTGACTCGGAT	AGAAGGGGGAAGAAACCTCA	50.0	50.0	58.0	57.9	181-187 bp	0.314607	63.0