Hotspot

ndhF

SSC · 109156-111417 · 2262 bp

Back to Scutellaria

Pi 0.0207
MarkerSeek score 64.10
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2262
variable_sites
468
pi
0.0207
unique_haplotype_count
150
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GCTCGGGTTATGGAAGAAGG GAGGTAAGGTCCGGTGAATC 55.0 55.0 58.0 57.4 2880-2965 bp 0.977528 83.2
ndhF_p2 AGGGCCCTCTTATCTCTTCC GAGGTAAGGTCCGGTGAATC 55.0 55.0 57.9 57.4 2908-2993 bp 0.977528 82.7
ndhF_p3 GGGCCCTCTTATCTCTTCCT GAGGTAAGGTCCGGTGAATC 55.0 55.0 57.9 57.4 2907-2992 bp 0.977528 82.7
ndhF_p4 TCGTTGTGGTCGGACTCTAT GAGGTAAGGTCCGGTGAATC 50.0 55.0 58.2 57.4 2954-2994 bp 0.97191 82.0
ndhF_p5 GCTCGGGTTATGGAAGAAGG TCAAAGGAGGTAAGGTCCGG 55.0 55.0 58.0 58.7 2912-2971 bp 0.786517 73.7