Hotspot

psaC-ndhE

SSC · 115656-115910 · 255 bp

Back to Scutellaria

Pi 0.0325

MarkerSeek score 67.80

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 255
variable_sites: 58
pi: 0.0325
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TACATCCGTCCCAGGGTATC	GCTATTGGACCGGCTATTGT	55.0	50.0	58.0	57.7	420-449 bp	0.983146	89.2
psaC-ndhE_p2	TACATCCGTCCCAGGGTATC	TAACTATTGCAGCCGCTGAA	55.0	45.0	58.0	57.6	443-472 bp	1.0	88.8
psaC-ndhE_p3	TCCGTCCCAGGGTATCATTT	GCTATTGGACCGGCTATTGT	50.0	50.0	58.1	57.7	416-445 bp	0.983146	88.4
psaC-ndhE_p4	ATCCGTCCCAGGGTATCATT	GCTATTGGACCGGCTATTGT	50.0	50.0	57.9	57.7	417-446 bp	0.983146	88.3
psaC-ndhE_p5	CTAATACATCGGTGGGGCAC	GCTATTGGACCGGCTATTGT	55.0	50.0	58.1	57.7	396-425 bp	0.97191	87.9