Hotspot

trnG-GCC-trnfM-CAU

LSC · 36660-36806 · 147 bp

Back to Scutellaria

Pi 0.0418
MarkerSeek score 70.30
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
147
variable_sites
44
pi
0.0418
unique_haplotype_count
111
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 242-292 bp 1.0 91.3
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 243-293 bp 1.0 91.3
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 206-256 bp 1.0 91.2
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 207-257 bp 1.0 91.2
trnG-GCC-trnfM-CAU_p5 ATGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 283-333 bp 1.0 91.0