Hotspot

trnT-GGU-psbD

LSC · 31743-33047 · 1305 bp

Back to Scutellaria

Pi 0.0163

MarkerSeek score 69.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1305
variable_sites: 270
pi: 0.0163
unique_haplotype_count: 131
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCGCAACCAGTCATCCATAA	55.0	50.0	58.1	58.0	1234-1583 bp	1.0	91.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.1	58.0	1237-1586 bp	1.0	91.0
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GACAAAAACGAAACGGTCCC	55.0	50.0	58.1	57.9	1255-1604 bp	1.0	90.6
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GACAAAAACGAAACGGTCCC	50.0	50.0	58.1	57.9	1258-1607 bp	1.0	90.4
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1254-1603 bp	1.0	89.5