Hotspot

rpoB-trnC-GCA

LSC · 27012-28171 · 1160 bp

Back to Scutellaria

Pi 0.0183

MarkerSeek score 68.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 228
pi: 0.0183
unique_haplotype_count: 124
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TTGTCTCATTTGCATCCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1044-1269 bp	0.988764	86.6
rpoB-trnC-GCA_p2	TTGTCTCATTTGCATCCCCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	58.4	1026-1251 bp	0.988764	84.4
rpoB-trnC-GCA_p3	GTCTCATTTGCATCCCCGAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.7	58.1	1042-1267 bp	0.966292	84.4
rpoB-trnC-GCA_p4	CAAACCCTGATCGATGAACCT	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.0	58.1	1112-1337 bp	1.0	83.3
rpoB-trnC-GCA_p5	GTCTCATTTGCATCCCCGAG	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.7	58.4	1024-1249 bp	0.966292	82.2