Hotspot

trnS-GCU-trnG-UCC

LSC · 8344-9043 · 700 bp

Back to Scutellaria

Pi 0.0207

MarkerSeek score 70.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 177
pi: 0.0207
unique_haplotype_count: 124
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AACCGAAAGACCCTTTAACT	50.0	40.0	58.0	54.5	665-887 bp	1.0	63.8
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AACCGAAAGACCCTTTAACT	50.0	40.0	58.0	54.5	664-886 bp	1.0	63.8
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	629-851 bp	1.0	63.7
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	628-850 bp	1.0	63.7
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCTTTAACT	50.0	40.0	58.2	54.5	719-941 bp	1.0	62.7