primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCCTTTCAGGATCAGTCGTG	AAAAGGGTATGTTGCTGCCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.023978	4256	5156	1392-1866	0.926966	88.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	AAAAGGGTATGTTGCTGCCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.023978	4256	5156	1391-1865	0.926966	88.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCCTTTCAGGATCAGTCGTG	CCCAATGAGCCGTTTATCGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.067632	4256	5156	882-1285	0.853933	85.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.067632	4256	5156	881-1284	0.853933	85.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTTTCAGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.067632	4256	5156	882-1285	0.853933	85.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6320	7064	723-1275	1.000000	90.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6320	7064	706-1258	1.000000	90.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	7.8	16.9	0.285346	6320	7064	724-1276	1.000000	89.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AGCACCCTCATTCCTAGACA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.7	58.1	0.0	16.9	0.330253	6320	7064	772-1324	1.000000	89.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6320	7064	720-1272	1.000000	88.4
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	AACCGAAAGACCCTTTAACT	20	20	50.0	40.0	58.0	54.5	0.0	0.0	3.519820	8344	9043	665-887	1.000000	63.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	AACCGAAAGACCCTTTAACT	20	20	50.0	40.0	58.0	54.5	0.0	0.0	3.521208	8344	9043	664-886	1.000000	63.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8344	9043	629-851	1.000000	63.7
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8344	9043	628-850	1.000000	63.7
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCTTTAACT	20	20	50.0	40.0	58.2	54.5	0.0	0.0	3.666586	8344	9043	719-941	1.000000	62.7
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TTGTCTCATTTGCATCCCCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.619512	27012	28171	1044-1269	0.988764	86.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTGTCTCATTTGCATCCCCG	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.5	58.4	0.0	14.6	0.892875	27012	28171	1026-1251	0.988764	84.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GTCTCATTTGCATCCCCGAG	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.7	58.1	0.0	0.0	0.778819	27012	28171	1042-1267	0.966292	84.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CAAACCCTGATCGATGAACCT	CCGGATTTGAACTGGGGAAA	21	20	47.6	50.0	58.0	58.1	0.5	0.0	1.090878	27012	28171	1112-1337	1.000000	83.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GTCTCATTTGCATCCCCGAG	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.7	58.4	0.0	14.6	1.052182	27012	28171	1024-1249	0.966292	82.2
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	CAGTCCCGACGGATCAAAAT	20	20	50.0	50.0	58.0	58.0	0.0	17.5	0.029546	29047	30062	1505-1810	1.000000	91.8
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	CAGTCCCGACGGATCAAAAT	20	20	50.0	50.0	58.0	58.0	0.0	17.5	0.029546	29047	30062	1503-1808	1.000000	91.8
petN-psbM_p3	petN-psbM	3	ACTCGTAGTGTGGGGAAGAA	GTCCCGACGGATCAAAATCA	20	20	50.0	50.0	58.0	58.0	0.0	17.5	0.023912	29047	30062	1503-1808	0.994382	91.6
petN-psbM_p4	petN-psbM	4	TCGTAGTGTGGGGAAGAAGT	GTCCCGACGGATCAAAATCA	20	20	50.0	50.0	58.0	58.0	0.0	17.5	0.023912	29047	30062	1501-1806	0.994382	91.6
petN-psbM_p5	petN-psbM	5	TCGTAGTGTGGGGAAGAAGT	AATTTTCTGCTCGATCCCCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.063963	29047	30062	1602-1907	0.617978	76.2
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	CCGCAACCAGTCATCCATAA	20	20	55.0	50.0	58.1	58.0	7.6	0.0	0.101025	31743	33047	1234-1583	1.000000	91.2
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	CCGCAACCAGTCATCCATAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.123180	31743	33047	1237-1586	1.000000	91.0
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	GACAAAAACGAAACGGTCCC	20	20	55.0	50.0	58.1	57.9	7.6	0.0	0.173676	31743	33047	1255-1604	1.000000	90.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	GACAAAAACGAAACGGTCCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.195831	31743	33047	1258-1607	1.000000	90.4
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	31743	33047	1254-1603	1.000000	89.5
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	36660	36806	242-292	1.000000	91.3
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	36660	36806	243-293	1.000000	91.3
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	36660	36806	206-256	1.000000	91.2
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	36660	36806	207-257	1.000000	91.2
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.124730	36660	36806	283-333	1.000000	91.0
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TCTCCTGAGGTTGTCGGAAT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.155778	44565	45447	502-1066	1.000000	90.8
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.212764	44565	45447	523-1087	1.000000	90.3
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.1	57.8	0.0	5.6	0.254079	44565	45447	473-1037	1.000000	90.0
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTCTCCTGAGGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.382307	44565	45447	503-1067	1.000000	88.9
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTCTCCTGAGGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.382307	44565	45447	504-1068	1.000000	88.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	GTCTACCGATTTCGCCACAT	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.196808	46830	47509	673-757	1.000000	90.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	GTTTCCCACATCAAGGCTCA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.223040	46830	47509	712-796	1.000000	90.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	GTCTACCGATTTCGCCACAT	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.304561	46830	47509	705-789	1.000000	89.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	GTTTCCCACATCAAGGCTCA	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.330794	46830	47509	744-828	1.000000	89.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	TCTACCGATTTCGCCACATC	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.351693	46830	47509	672-756	1.000000	89.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCAAAATAGGAATAAGACTTGA	CCGAGAAAGTCTACGGTTCG	24	20	33.3	55.0	56.3	58.1	0.0	0.0	5.764085	50764	51748	550-1128	1.000000	52.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCAAAATAGGAATAAGACTTG	CCGAGAAAGTCTACGGTTCG	23	20	34.8	55.0	54.9	58.1	0.0	0.0	6.163964	50764	51748	550-1128	1.000000	52.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ATGCCAAAATAGGAATAAGACTTGA	CCGAGAAAGTCTACGGTTCG	25	20	32.0	55.0	56.8	58.1	0.0	0.0	6.305861	50764	51748	551-1129	1.000000	52.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCAAAATAGGAATAAGACTTGAT	CCGAGAAAGTCTACGGTTCG	25	20	32.0	55.0	56.8	58.1	0.0	0.0	6.305861	50764	51748	550-1128	1.000000	52.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAAATAGGAATAAGACTTGA	GTTCGAGTCCGTATAGCCCT	24	20	33.3	55.0	56.3	58.7	0.0	0.0	6.339901	50764	51748	535-1113	0.994382	51.8
rbcL-accD_p1	rbcL-accD	1	TTGAATGCTACTGCGGGTAC	ACTATTCTTCGCGTGGGTTC	20	20	50.0	50.0	58.0	58.0	0.0	5.8	0.024512	56965	57677	2030-2088	0.983146	91.1
rbcL-accD_p2	rbcL-accD	2	TCATGGTATGCACTTCCGTG	ACTATTCTTCGCGTGGGTTC	20	20	50.0	50.0	58.0	58.0	15.5	5.8	0.028735	56965	57677	1830-1888	0.983146	91.1
rbcL-accD_p3	rbcL-accD	3	GAGGCTAGCAAATGGAGTCC	ACTATTCTTCGCGTGGGTTC	20	20	55.0	50.0	58.0	58.0	18.2	5.8	0.045554	56965	57677	1409-1467	0.983146	91.0
rbcL-accD_p4	rbcL-accD	4	TCTTCACATTCACCGTGCAA	ACTATTCTTCGCGTGGGTTC	20	20	45.0	50.0	58.0	58.0	0.0	5.8	0.045554	56965	57677	1878-1936	0.983146	91.0
rbcL-accD_p5	rbcL-accD	5	TTCTTCACATTCACCGTGCA	ACTATTCTTCGCGTGGGTTC	20	20	45.0	50.0	58.0	58.0	0.0	5.8	0.045554	56965	57677	1879-1937	0.983146	91.0
petA-psbJ_p1	petA-psbJ	1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	63478	64484	821-1124	1.000000	86.7
petA-psbJ_p2	petA-psbJ	2	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	63478	64484	820-1123	1.000000	86.2
petA-psbJ_p3	petA-psbJ	3	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	20	20	45.0	50.0	57.7	57.5	5.4	0.0	0.775892	63478	64484	820-1123	1.000000	85.8
petA-psbJ_p4	petA-psbJ	4	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	20	20	50.0	50.0	58.2	57.5	5.4	0.0	0.668311	63478	64484	823-1126	0.926966	83.7
petA-psbJ_p5	petA-psbJ	5	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	20	20	50.0	55.0	58.2	57.4	5.4	0.0	0.729614	63478	64484	822-1125	0.926966	83.2
rpl20-rps12_p1	rpl20-rps12	1	TGAATGAGACCCCCGAAAAC	ACAGCCCATTCGAAATGTCA	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.393028	69147	69940	952-975	0.994382	88.4
rpl20-rps12_p2	rpl20-rps12	2	ATGAGACCCCCGAAAACTTG	ACAGCCCATTCGAAATGTCA	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.399200	69147	69940	949-972	0.994382	88.3
rpl20-rps12_p3	rpl20-rps12	3	TGAATGAGACCCCCGAAAAC	ACAAGACAGCCCATTCGAAA	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.464553	69147	69940	957-980	1.000000	88.0
rpl20-rps12_p4	rpl20-rps12	4	ATGAGACCCCCGAAAACTTG	ACAAGACAGCCCATTCGAAA	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.470725	69147	69940	954-977	1.000000	88.0
rpl20-rps12_p5	rpl20-rps12	5	CGGGCTATATATCCGCGTTT	ACAGCCCATTCGAAATGTCA	20	20	50.0	45.0	57.7	57.8	4.9	0.0	0.487788	69147	69940	897-920	0.994382	87.8
ndhF_p1	ndhF	1	GCTCGGGTTATGGAAGAAGG	GAGGTAAGGTCCGGTGAATC	20	20	55.0	55.0	58.0	57.4	0.0	0.0	0.643759	109156	111417	2880-2965	0.977528	83.2
ndhF_p2	ndhF	2	AGGGCCCTCTTATCTCTTCC	GAGGTAAGGTCCGGTGAATC	20	20	55.0	55.0	57.9	57.4	22.3	0.0	0.709439	109156	111417	2908-2993	0.977528	82.7
ndhF_p3	ndhF	3	GGGCCCTCTTATCTCTTCCT	GAGGTAAGGTCCGGTGAATC	20	20	55.0	55.0	57.9	57.4	17.0	0.0	0.709439	109156	111417	2907-2992	0.977528	82.7
ndhF_p4	ndhF	4	TCGTTGTGGTCGGACTCTAT	GAGGTAAGGTCCGGTGAATC	20	20	50.0	55.0	58.2	57.4	8.2	0.0	0.768407	109156	111417	2954-2994	0.971910	82.0
ndhF_p5	ndhF	5	GCTCGGGTTATGGAAGAAGG	TCAAAGGAGGTAAGGTCCGG	20	20	55.0	55.0	58.0	58.7	0.0	0.0	0.772266	109156	111417	2912-2971	0.786517	73.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGTACGACAAACGAATAAACT	TCCTAAGAGCAGCGTGTCTA	21	20	33.3	50.0	53.6	57.9	0.0	0.0	5.554971	111922	112367	334-699	1.000000	52.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGTACGACAAACGAATAAACT	AGCAGCGTGTCTACCAATTT	21	20	33.3	45.0	53.6	57.8	0.0	0.0	5.615698	111922	112367	327-692	1.000000	52.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGTACGACAAACGAATAAACT	CCTAAGAGCAGCGTGTCTAC	21	20	33.3	55.0	53.6	57.8	0.0	0.0	5.626989	111922	112367	333-698	1.000000	52.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGTACGACAAACGAATAAACT	CTAAGAGCAGCGTGTCTACC	21	20	33.3	55.0	53.6	57.8	0.0	0.0	5.626989	111922	112367	332-697	1.000000	52.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGTACGACAAACGAATAAACT	GAGCAGCGTGTCTACCAATT	21	20	33.3	50.0	53.6	58.3	0.0	0.0	5.703840	111922	112367	328-693	1.000000	52.0
psaC-ndhE_p1	psaC-ndhE	1	TACATCCGTCCCAGGGTATC	GCTATTGGACCGGCTATTGT	20	20	55.0	50.0	58.0	57.7	5.0	0.0	0.261487	115656	115910	420-449	0.983146	89.2
psaC-ndhE_p2	psaC-ndhE	2	TACATCCGTCCCAGGGTATC	TAACTATTGCAGCCGCTGAA	20	20	55.0	45.0	58.0	57.6	5.0	24.8	0.400300	115656	115910	443-472	1.000000	88.8
psaC-ndhE_p3	psaC-ndhE	3	TCCGTCCCAGGGTATCATTT	GCTATTGGACCGGCTATTGT	20	20	50.0	50.0	58.1	57.7	5.0	0.0	0.368320	115656	115910	416-445	0.983146	88.4
psaC-ndhE_p4	psaC-ndhE	4	ATCCGTCCCAGGGTATCATT	GCTATTGGACCGGCTATTGT	20	20	50.0	50.0	57.9	57.7	5.0	0.0	0.374521	115656	115910	417-446	0.983146	88.3
psaC-ndhE_p5	psaC-ndhE	5	CTAATACATCGGTGGGGCAC	GCTATTGGACCGGCTATTGT	20	20	55.0	50.0	58.1	57.7	0.0	0.0	0.377164	115656	115910	396-425	0.971910	87.9
ycf1_p1	ycf1	1	GCCTTTCCATTGACTCGGAT	GCTCGGGTTATGGAAGAAGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.089196	126418	126537	680-815	0.837079	84.8
ycf1_p2	ycf1	2	GCCTTTCCATTGACTCGGAT	AGGGCCCTCTTATCTCTTCC	20	20	50.0	55.0	58.0	57.9	0.0	22.3	0.154876	126418	126537	708-843	0.837079	84.2
ycf1_p3	ycf1	3	GCCTTTCCATTGACTCGGAT	GGGCCCTCTTATCTCTTCCT	20	20	50.0	55.0	58.0	57.9	0.0	17.0	0.154876	126418	126537	707-842	0.837079	84.2
ycf1_p4	ycf1	4	GCCTTTCCATTGACTCGGAT	TCGTTGTGGTCGGACTCTAT	20	20	50.0	50.0	58.0	58.2	0.0	8.2	0.213844	126418	126537	754-889	0.837079	83.8
ycf1_p5	ycf1	5	GCCTTTCCATTGACTCGGAT	AGAAGGGGGAAGAAACCTCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.177981	126418	126537	181-187	0.314607	63.0