Hotspot

ycf1

SSC · 95251-100311 · 5061 bp

Back to Scurrula

Pi 0.0422

MarkerSeek score 75.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5061
variable_sites: 608
pi: 0.0422
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTGACAAGGAAGATAAGCCA	CTTTTGGTCCTCCCCGAAAA	42.9	50.0	56.9	58.0	805-832 bp	1.0	75.4
ycf1_p2	TGTGACAAGGAAGATAAGCCA	TTTGGTCCTCCCCGAAAAAG	42.9	50.0	56.9	58.0	803-830 bp	1.0	75.4
ycf1_p3	TGACAGTTTTGATCTGACACGA	CTTTTGGTCCTCCCCGAAAA	40.9	50.0	57.9	58.0	1207-1213 bp	0.571429	50.7
ycf1_p4	TGACAGTTTTGATCTGACACGA	TTTGGTCCTCCCCGAAAAAG	40.9	50.0	57.9	58.0	1205-1211 bp	0.571429	50.7
ycf1_p5	TGACAGTTTTGATCTGACACGA	TCCCGAATGGTCTGAGGATT	40.9	50.0	57.9	58.1	1467-1476 bp	0.571429	49.5